Active Recombinant Human CSF3, His-tagged

• Cat.No.: CSF3-21H
• Product Overview: Recombinant Human CSF3 was purified from the endosperm tissue of barley grain (Hordeum vulgare). Recombinant human G-CSF is a 18.7 kDa protein containing 174 amino acid residues.

Specification

• Species: Human
• Source: Barley Grain
• Tag: His
• Description: Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive.
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
• Bio-activity: Recombinant human G-CSF was determined by its dose-dependent effects on the proliferation of M-NFS-60 cells. The ED50 value is typically< 0.1="" ng/ml="" corresponding="" to="" specific="" activity="">1 x 10e7 U/mg. Optimal concentration should be determined for specific applications and cell lines.
• Molecular Mass: Recombinant human G-CSF contains 174 amino acids and a 16 a.a. Histidine-based tag for a total length of 190 a.a. and has a predicted molecular mass of 20.8 kDa.
• Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
• Purity: Greater than 95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3 (granulocyte); C17orf33, chromosome 17 open reading frame 33 , G CSF, GCSF; granulocyte colony-stimulating factor; filgrastim; granulocyte colony stimulating factor; lenograstim; MGC45931; pluripoietin; GCSF; CSF3OS; C17orf33
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• UniProt ID: P09919
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity