Active Recombinant Human CSF3, His-tagged
Cat.No. : | CSF3-21H |
Product Overview : | Recombinant Human CSF3 was purified from the endosperm tissue of barley grain (Hordeum vulgare). Recombinant human G-CSF is a 18.7 kDa protein containing 174 amino acid residues. |
- Specification
- Gene Information
- Related Products
Description : | Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. |
Source : | Barley grain (Hordeum vulgare) |
Species : | Human |
Tag : | His |
Form : | Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2 |
Bio-activity : | Recombinant human G-CSF was determined by its dose-dependent effects on the proliferation of M-NFS-60 cells. The ED50 value is typically< 0.1 ng/ml corresponding to specific activity>1 x 10e7 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
Molecular Mass : | Recombinant human G-CSF contains 174 amino acids and a 16 a.a. Histidine-based tag for a total length of 190 a.a. and has a predicted molecular mass of 20.8 kDa. |
Endotoxin : | Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg). |
Purity : | Greater than 95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ] |
Official Symbol : | CSF3 |
Synonyms : | CSF3; colony stimulating factor 3 (granulocyte); C17orf33, chromosome 17 open reading frame 33 , G CSF, GCSF; granulocyte colony-stimulating factor; filgrastim; granulocyte colony stimulating factor; lenograstim; MGC45931; pluripoietin; GCSF; CSF3OS; C17orf33; |
Gene ID : | 1440 |
mRNA Refseq : | NM_000759 |
Protein Refseq : | NP_000750 |
MIM : | 138970 |
UniProt ID : | P09919 |
Chromosome Location : | 17q11.2-q12 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; |
Function : | cytokine activity; cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity; |
Products Types
◆ Recombinant Protein | ||
CSF3-49H | Active Recombinant Human CSF3 Protein | +Inquiry |
CSF3-16H | Active Recombinant Human CSF3 Protein, Animal Free | +Inquiry |
CSF3-1165M | Recombinant Mouse CSF3 protein(Met1-Ala208) | +Inquiry |
Csf3-2163R | Recombinant Rat Csf3 Protein, His-tagged | +Inquiry |
CSF3-3348H | Recombinant Human CSF3 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CSF3-2948HCL | Recombinant Human CSF3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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