Active Recombinant Human CSF3 (27 - 200aa), Met tagged

• Cat.No.: CSF3-024E
• Product Overview: Recombinant Human CSF3 (Thr27 ­ Pro200, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Cat. No.: CSF3-024E
• Description: G­CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF­μ and IFN­μ. In vitro, G­CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL­3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G­CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
• Source: E. coli
• N­terminal Sequence Analysis: Met
• Molecular Mass: 18.8 kDa
• Activity: Measured in a cell proliferation assay using NFS­60 mouse myeloid cells. The ED50 for this effect is typically 10 ­ 60 pg/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetic Acid with Trehalose. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 3 month, 2 to 8 °C under sterile conditions after reconstitution.
• Unitprot ID: P09919

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3 (granulocyte); GCSF; CSF3OS; C17orf33; MGC45931; granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity