Recombinant Mouse FGFR2 cell lysate
Cat.No. : | FGFR2-001MCL |
Product Overview : | Mouse FGFR2 / CD332 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Fgfr2 fibroblast growth factor receptor 2 [ Mus musculus ] |
Official Symbol : | FGFR2 |
Synonyms : | FGFR2; fibroblast growth factor receptor 2; keratinocyte growth factor receptor; Bek; svs; KGFR; Fgfr7; Fgfr-2; Fgfr-7; KGFRTr; AU043015; AW556123; MGC102519; |
Gene ID : | 14183 |
mRNA Refseq : | NM_010207 |
Protein Refseq : | NP_034337 |
Pathway : | Downstream signaling of activated FGFR, organism-specific biosystem; ESC Pluripotency Pathways, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR2 ligand binding and activation, organism-specific biosystem; FGFR2b ligand binding and activation, organism-specific biosystem; |
Function : | ATP binding; fibroblast growth factor 1 binding; fibroblast growth factor binding; fibroblast growth factor binding; fibroblast growth factor-activated receptor activity; heparin binding; kinase activity; nucleotide binding; protein binding; protein homodimerization activity; protein kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity; |
Products Types
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◆ Lysates | ||
FGFR2-2578HCL | Recombinant Human FGFR2 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0341 | FGFR2 Kinase (Human) Assay/Inhibitor Screening Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionMutations in FGFR2 are associated with various developmental disorders, impacting the development of the skull, limbs, and other structures.
Yes, targeting FGFR2 mutations allows for more personalized and precise therapeutic approaches in cancer treatment.
Yes, numerous clinical trials are underway to evaluate the efficacy of FGFR2 inhibitors in treating various cancers and developmental disorders.
FGFR2 is involved in the regulation of cell migration and tissue repair, contributing to the process of wound healing.
Common side effects of FGFR2 inhibitors include fatigue, nausea, and hypertension, which are being studied for better management.
Customer Reviews (3)
Write a reviewThe FGFR2 protein is an outstanding product that possesses exceptional quality, making it an ideal choice for my experimental needs.
What sets FGFR2 apart is the manufacturer's unparalleled technical support.
The versatility of the FGFR2 protein makes it a valuable asset in my research.
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