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Recombinant Canine ANGPTL1 cell lysate

Cat.No. : ANGPTL1-001CCL
Product Overview : Canine ANGPTL1 / Angiopoietin-like 1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Canine
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : ANGPTL1 angiopoietin-like 1 [ Canis lupus familiaris ]
Official Symbol : ANGPTL1
Synonyms : angiopoietin-related protein 1
Gene ID : 612843
mRNA Refseq : NM_001109813
Protein Refseq : NP_001103283
Chromosome Location : chromosome: 7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can ANGPTL1 be targeted for therapeutic purposes? 11/16/2022

Targeting ANGPTL1 for therapeutic purposes is an area of ongoing research. Due to its involvement in angiogenesis and adipogenesis, regulating ANGPTL1 levels or activity could have potential therapeutic implications in conditions such as cancer, obesity, and metabolic disorders. Developing specific ANGPTL1-targeted therapies, such as antibodies or small molecules, could provide potential treatment options. However, more research is necessary to fully understand the therapeutic potential of targeting ANGPTL1.

How is ANGPTL1 involved in adipogenesis? 09/07/2021

ANGPTL1 plays a role in adipogenesis, the process of fat cell development. It promotes the differentiation of preadipocytes, which are precursor cells for fat cells, into mature adipocytes. ANGPTL1 accomplishes this by binding to the α5β1 integrin receptor on preadipocytes. This interaction activates downstream signaling pathways that induce adipocyte differentiation and lipid accumulation.

Can ANGPTL1 affect glucose metabolism? 12/11/2020

Yes, ANGPTL1 has been implicated in the regulation of glucose metabolism. Studies have shown that ANGPTL1 expression is upregulated in response to insulin and high glucose levels. It has been suggested that ANGPTL1 may contribute to insulin sensitivity and glucose homeostasis by enhancing insulin signaling pathways and glucose uptake in specific tissues. However, the precise mechanisms through which ANGPTL1 affects glucose metabolism are still under investigation.

Can ANGPTL1 be used as a biomarker for any diseases? 08/26/2018

ANGPTL1 has been investigated as a potential biomarker for various diseases. For example, elevated levels of ANGPTL1 have been observed in the plasma of patients with certain types of cancer, such as colorectal cancer, ovarian cancer, and pancreatic cancer. It has also been suggested as a biomarker for kidney diseases, where increased levels of ANGPTL1 have been associated with renal function decline.

Are there any therapeutic implications of ANGPTL1? 08/14/2018

The inhibition or manipulation of ANGPTL1 has been considered as a potential therapeutic strategy in various contexts. In diseases where excessive angiogenesis is detrimental, such as cancer, inhibiting ANGPTL1 could potentially be utilized to restrict tumor vascularization and growth. Conversely, in conditions where promoting angiogenesis is beneficial, such as tissue repair and wound healing, boosting ANGPTL1 activity might be beneficial.

Is ANGPTL1 associated with any diseases or conditions? 11/18/2017

ANGPTL1 has been studied in relation to several diseases and conditions. Studies have suggested that it may be involved in the pathogenesis of cancers, such as breast cancer and liver cancer, where it can promote tumor growth and metastasis by stimulating angiogenesis. Furthermore, ANGPTL1 expression has been found to be altered in obesity and metabolic disorders, suggesting a potential role in metabolic regulation. However, more research is needed to fully understand the role of ANGPTL1 in these diseases and conditions.

Are there any known genetic variations or mutations in the ANGPTL1 gene? 04/23/2017

Genetic variations and mutations in the ANGPTL1 gene have been reported in various studies. Some genetic variants have been associated with altered ANGPTL1 expression levels or activity and have been linked to certain diseases or conditions. For example, single nucleotide polymorphisms (SNPs) in the ANGPTL1 gene have been associated with coronary artery disease and susceptibility to gestational diabetes mellitus.

Are there any known inhibitors or activators of ANGPTL1? 07/29/2016

Currently, there is limited information available on specific inhibitors or activators of ANGPTL1. Research in this area is still ongoing. However, modulating the expression of ANGPTL1 may be possible through various factors, such as growth factors, cytokines, or signaling pathways that can regulate the expression of angiopoietin-like proteins. Further studies are needed to identify potential regulators of ANGPTL1 activity.

How is ANGPTL1 involved in angiogenesis? 05/16/2016

ANGPTL1 is known to inhibit angiogenesis, the process of forming new blood vessels. It can interfere with the activity of angiogenic factors like vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF), thus suppressing blood vessel formation. ANGPTL1 can compete with angiopoietins, which are crucial for blood vessel development, for binding to their receptors, Tie2 and Tie1. By doing so, ANGPTL1 disrupts the angiopoietin-Tie signaling pathway, inhibiting angiogenesis.

Can ANGPTL1 be used as a diagnostic marker for certain diseases? 03/18/2016

It is not currently known if ANGPTL1 can be used as a diagnostic marker for specific diseases. However, studies have indicated its potential as a biomarker in certain conditions. For instance, increased levels of ANGPTL1 have been associated with adverse vascular outcomes in patients with coronary artery disease. Nevertheless, further research is needed to validate ANGPTL1 as a reliable diagnostic marker for specific diseases.

Customer Reviews (5)

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Reviews
03/25/2023

    One of the primary advantages of the ANGPTL1 protein is its versatility in various trial applications.

    10/28/2019

      As a researcher utilizing the ANGPTL1 protein in my trials, I have been highly impressed with its numerous advantages that significantly contribute to the success of my experimental endeavors.

      10/16/2018

        It can be effectively employed in in vitro and in vivo studies, offering insights into the intricate mechanisms underlying angiogenesis, lipid metabolism, and cardiovascular diseases.

        05/12/2017

          Its purity and consistency enable accurate and precise data analysis, ensuring the integrity of my research outcomes.

          10/29/2016

            the ANGPTL1 protein's exceptional quality, reliability, and the manufacturer's unparalleled technical support make it a prime choice for researchers seeking to delve into the intricacies of angiogenesis, vascular development, and related fields.

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