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Recombinant Human PDHA1 cell lysate

Cat.No. : PDHA1-001HCL
Product Overview : Human PDHA1 / C54G1 (aa 30-390) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 [ Homo sapiens ]
Official Symbol : PDHA1
Synonyms : PDHA1; pyruvate dehydrogenase (lipoamide) alpha 1; PDHA; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; PHE1A; PDHCE1A;
Gene ID : 5160
mRNA Refseq : NM_000284
Protein Refseq : NP_000275
MIM : 300502
UniProt ID : P08559
Chromosome Location : Xp22.1
Pathway : Acetylcholine Synthesis, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem;
Function : oxidoreductase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor; pyruvate dehydrogenase (acetyl-transferring) activity; pyruvate dehydrogenase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the symptoms of PDHA1-related disorders? 01/28/2022

Symptoms may include developmental delay, intellectual disability, muscle weakness, and movement disorders.

What are the treatment options for PDHA1-related disorders? 12/01/2020

Treatment may involve dietary modifications, supplements, and supportive therapies to manage symptoms.

Are there any ongoing research efforts related to PDHA1 protein? 07/17/2019

Yes, researchers are studying potential gene therapy approaches and novel treatments for PDHA1-related disorders.

How does the PDHA1 protein impact brain function? 01/05/2018

The PDHA1 protein is critical for supplying energy to the brain, and mutations can lead to neurological impairments.

How does a mutation in the PDHA1 gene affect human health? 12/25/2017

Mutations in the PDHA1 gene can lead to pyruvate dehydrogenase complex deficiency, resulting in neurological and developmental problems.

Customer Reviews (3)

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Reviews
06/27/2020

    Incorporating the PDHA1 protein into your research will not only meet but exceed your expectations, enabling you to advance your scientific discoveries with confidence.

    02/28/2019

      Researchers can leverage the unique properties of the PDHA1 protein to gain insights into its biological functions and its impact on disease mechanisms.

      07/10/2017

        It has been shown to play a crucial role in multiple cellular processes, making it highly valuable for investigations into cell signaling, inflammation, and immune responses.

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