Recombinant Mouse MAP2K1 cell lysate
Cat.No. : | MAP2K1-001MCL |
Product Overview : | Mouse MAP2K1 / MEK1 / MAPKK1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Map2k1 mitogen-activated protein kinase kinase 1 [ Mus musculus ] |
Official Symbol : | MAP2K1 |
Synonyms : | MAP2K1; mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; mitogen activated protein kinase kinase 1; protein kinase, mitogen activated, kinase 1, p45; Mek1; MEKK1; MAPKK1; Prkmk1; |
Gene ID : | 26395 |
mRNA Refseq : | NM_008927 |
Protein Refseq : | NP_032953 |
Pathway : | ARMS-mediated activation, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; |
Function : | ATP binding; MAP kinase kinase activity; MAP kinase kinase activity; Ras GTPase binding; kinase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine phosphatase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; |
Products Types
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MAP2K1-1310M | Recombinant Mouse MAP2K1 protein(Met1-Ile393), His&GST-tagged | +Inquiry |
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◆ Assay kits | ||
Kit-1614 | MEK1 Kinase Binding Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, there are clinical trials exploring the efficacy and safety of MAP2K1 inhibitors in various cancers and other diseases.
Some diagnostic tests may analyze MAP2K1 mutations, especially in the context of cancer, to inform treatment decisions.
MAP2K1 mutations can serve as biomarkers in cancer diagnostics, aiding in the identification and classification of tumors.
MAP2K1 mutations can be associated with resistance to certain cancer therapies, making it important to understand its role in treatment response.
Yes, MAP2K1 inhibitors are being explored as targeted therapies in cancer treatment, aiming to block the abnormal signaling associated with MAP2K1 mutations.
Customer Reviews (3)
Write a reviewThe unmatched performance of the MAP2K1 protein in both ELISA assays and protein electron microscopy structure analysis showcases its versatility and reliability in various experimental settings.
The MAP2K1 protein finds extensive utility in protein electron microscopy structure analysis.
With its exceptional specificity and sensitivity, it enables precise and reliable detection of target molecules, making it a valuable tool in the field of diagnostic and research applications.
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