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Recombinant Human HDAC4 cell lysate

Cat.No. : HDAC4-001HCL
Product Overview : Human HDAC4 (aa 612-1084) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : HDAC4 histone deacetylase 4 [ Homo sapiens ]
Official Symbol : HDAC4
Synonyms : HDAC4; histone deacetylase 4; HA6116; HD4; HDAC 4; HDAC A; HDACA; KIAA0288; histone deacetylase A; AHO3; BDMR; HDAC-4; HDAC-A;
Gene ID : 9759
mRNA Refseq : NM_006037
Protein Refseq : NP_006028
MIM : 605314
UniProt ID : P56524
Chromosome Location : 2q37.3
Pathway : Cell cycle, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem;
Function : NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); activating transcription factor binding; histone deacetylase activity; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; potassium ion binding; protein binding; protein deacetylase activity; protein kinase binding; repressing transcription factor binding; contributes_to sequence-specific DNA binding; transcription factor binding; contributes_to transcription regulatory region DNA binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the impact of HDAC4 on cardiac hypertrophy and heart failure pathogenesis? 12/03/2022

HDAC4 affects cardiac hypertrophy and heart failure by regulating cardiac gene expression.

What are the mechanisms by which HDAC4 modulates immune responses and inflammation? 06/14/2022

HDAC4 regulates immune responses and inflammation through gene expression control.

What role does HDAC4 play in neuronal development and neurodegenerative diseases? 10/10/2020

HDAC4 is important for neuronal development and linked to neurodegenerative diseases.

How do environmental factors like stress and diet influence the activity and regulation of HDAC4? 03/29/2020

Stress and diet influence HDAC4 activity, affecting its role in gene regulation and cell processes.

How do mutations in the HDAC4 gene contribute to skeletal muscle development and related disorders? 03/16/2020

Mutations in HDAC4 disrupt skeletal muscle development and contribute to muscular disorders.

How does HDAC4 regulate gene expression through histone modification in different cell types? 04/01/2019

HDAC4 controls gene expression by altering histone acetylation, affecting chromatin structure.

How does HDAC4 interact with non-histone proteins and influence their function? 07/19/2018

HDAC4 interacts with non-histone proteins, influencing various cellular functions.

Customer Reviews (3)

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Reviews
04/29/2022

    Dependable protein-protein interaction assays; integral for our experiments.

    12/23/2021

      Efficient protein purification services; consistent yield and purity.

      12/19/2018

        Expertise in protein engineering; excellent service, highly recommended.

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