Recombinant Human HDAC4 cell lysate
Cat.No. : | HDAC4-001HCL |
Product Overview : | Human HDAC4 (aa 612-1084) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | HDAC4 histone deacetylase 4 [ Homo sapiens ] |
Official Symbol : | HDAC4 |
Synonyms : | HDAC4; histone deacetylase 4; HA6116; HD4; HDAC 4; HDAC A; HDACA; KIAA0288; histone deacetylase A; AHO3; BDMR; HDAC-4; HDAC-A; |
Gene ID : | 9759 |
mRNA Refseq : | NM_006037 |
Protein Refseq : | NP_006028 |
MIM : | 605314 |
UniProt ID : | P56524 |
Chromosome Location : | 2q37.3 |
Pathway : | Cell cycle, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem; |
Function : | NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); activating transcription factor binding; histone deacetylase activity; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; potassium ion binding; protein binding; protein deacetylase activity; protein kinase binding; repressing transcription factor binding; contributes_to sequence-specific DNA binding; transcription factor binding; contributes_to transcription regulatory region DNA binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
HDAC4-1044H | Recombinant Human HDAC4 Protein (T648-T1057), Tag Free | +Inquiry |
HDAC4-4645H | Recombinant Human HDAC4 Protein, GST-tagged | +Inquiry |
HDAC4-022H | Recombinant Human HDAC4 Protein, GST-tagged | +Inquiry |
HDAC4-2472R | Recombinant Rat HDAC4 Protein, His (Fc)-Avi-tagged | +Inquiry |
HDAC4-1045H | Recombinant Human HDAC4 Protein (T648-T1057), His tagged | +Inquiry |
◆ Assay kits | ||
Kit-1740 | HDAC4 Fluorogenic Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionHDAC4 affects cardiac hypertrophy and heart failure by regulating cardiac gene expression.
HDAC4 regulates immune responses and inflammation through gene expression control.
HDAC4 is important for neuronal development and linked to neurodegenerative diseases.
Stress and diet influence HDAC4 activity, affecting its role in gene regulation and cell processes.
Mutations in HDAC4 disrupt skeletal muscle development and contribute to muscular disorders.
HDAC4 controls gene expression by altering histone acetylation, affecting chromatin structure.
HDAC4 interacts with non-histone proteins, influencing various cellular functions.
Customer Reviews (3)
Write a reviewDependable protein-protein interaction assays; integral for our experiments.
Efficient protein purification services; consistent yield and purity.
Expertise in protein engineering; excellent service, highly recommended.
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