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Recombinant Human ACOX1 cell lysate

Cat.No.: ACOX1-511HCL
Product Overview: Human ACOX1 / aox derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Baculovirus-Insect Cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: ACOX1 acyl-CoA oxidase 1, palmitoyl [ Homo sapiens ]
Official Symbol: ACOX1
Synonyms: ACOX1; acyl-CoA oxidase 1, palmitoyl; acyl Coenzyme A oxidase 1, palmitoyl; peroxisomal acyl-coenzyme A oxidase 1; PALMCOX; AOX; palmitoyl-CoA oxidase; straight-chain acyl-CoA oxidase; acyl-CoA oxidase, straight-chain; peroxisomal fatty acyl-CoA oxidase; acyl-Coenzyme A oxidase 1, palmitoyl; ACOX; SCOX; MGC1198;
Gene ID: 51
mRNA Refseq: NM_001185039
Protein Refseq: NP_001171968
MIM: 609751
UniProt ID: Q15067
Chromosome Location: 17q25.1
Pathway: Beta-oxidation of very long chain fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: acyl-CoA dehydrogenase activity; acyl-CoA oxidase activity; acyl-CoA oxidase activity; acyl-CoA oxidase activity; flavin adenine dinucleotide binding; protein N-terminus binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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