Recombinant Mouse PRKCD cell lysate
Cat.No. : | PRKCD-546MCL |
Product Overview : | Mouse PKC delta / PRKCD derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Prkcd protein kinase C, delta [ Mus musculus ] |
Official Symbol : | PRKCD |
Synonyms : | PRKCD; protein kinase C, delta; protein kinase C delta type; nPKC-delta; protein kinase C[d]; protein kinase C, delta V; protein kinase C, delta IV; tyrosine-protein kinase PRKCD; protein kinase C delta variant IX; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e; |
Gene ID : | 18753 |
mRNA Refseq : | NM_011103 |
Protein Refseq : | NP_035233 |
Pathway : | Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; |
Function : | ATP binding; TIR domain binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; insulin receptor substrate binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein C-terminus binding; protein kinase C activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionLike any targeted therapy, there may be side effects associated with PRKCD inhibitors, and ongoing clinical trials aim to assess the safety profile of these potential treatments.
Yes, PRKCD is implicated in the regulation of inflammation, and its modulation could be explored for the development of anti-inflammatory therapies.
The role of PRKCD in various diseases suggests that it could be a potential target for personalized medicine, tailoring treatments based on an individual's molecular profile.
PRKCD has been found to influence immune response, and its modulation could potentially be harnessed to enhance the body's ability to fight off infections or respond to immunotherapies.
Research suggests that the expression levels of PRKCD may serve as a biomarker for certain cancers, aiding in early diagnosis and prognosis.
Customer Reviews (3)
Write a reviewTheir knowledgeable and responsive team has consistently demonstrated their ability to address any concerns or challenges I have encountered along the way.
Its versatility allows for exploring its various roles and functions, shedding light on its significance in crucial biochemical pathways and cellular processes.
Their support has been invaluable in troubleshooting and providing efficient solutions, enhancing the overall efficiency and productivity of my experiments.
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