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Recombinant Mouse PRKCD cell lysate

Cat.No. : PRKCD-546MCL
Product Overview : Mouse PKC delta / PRKCD derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Prkcd protein kinase C, delta [ Mus musculus ]
Official Symbol : PRKCD
Synonyms : PRKCD; protein kinase C, delta; protein kinase C delta type; nPKC-delta; protein kinase C[d]; protein kinase C, delta V; protein kinase C, delta IV; tyrosine-protein kinase PRKCD; protein kinase C delta variant IX; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e;
Gene ID : 18753
mRNA Refseq : NM_011103
Protein Refseq : NP_035233
Pathway : Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem;
Function : ATP binding; TIR domain binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; insulin receptor substrate binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein C-terminus binding; protein kinase C activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there any side effects associated with targeting PRKCD in therapy? 10/19/2021

Like any targeted therapy, there may be side effects associated with PRKCD inhibitors, and ongoing clinical trials aim to assess the safety profile of these potential treatments.

Is PRKCD involved in the regulation of inflammation? 11/22/2019

Yes, PRKCD is implicated in the regulation of inflammation, and its modulation could be explored for the development of anti-inflammatory therapies.

Can PRKCD be a target for personalized medicine? 09/25/2019

The role of PRKCD in various diseases suggests that it could be a potential target for personalized medicine, tailoring treatments based on an individual's molecular profile.

How does PRKCD impact immune response? 11/22/2017

PRKCD has been found to influence immune response, and its modulation could potentially be harnessed to enhance the body's ability to fight off infections or respond to immunotherapies.

Can PRKCD be a potential biomarker for cancer diagnosis? 07/05/2016

Research suggests that the expression levels of PRKCD may serve as a biomarker for certain cancers, aiding in early diagnosis and prognosis.

Customer Reviews (3)

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Reviews
09/12/2021

    Their knowledgeable and responsive team has consistently demonstrated their ability to address any concerns or challenges I have encountered along the way.

    04/11/2020

      Its versatility allows for exploring its various roles and functions, shedding light on its significance in crucial biochemical pathways and cellular processes.

      06/17/2018

        Their support has been invaluable in troubleshooting and providing efficient solutions, enhancing the overall efficiency and productivity of my experiments.

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