Recombinant Human STAT1 cell lysate
Cat.No. : | STAT1-555HCL |
Product Overview : | Human STAT1 / p91 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | STAT1 signal transducer and activator of transcription 1, 91kDa [ Homo sapiens ] |
Official Symbol : | STAT1 |
Synonyms : | STAT1; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1-alpha/beta; ISGF 3; STAT91; transcription factor ISGF 3 components p91/p84; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; CANDF7; ISGF-3; DKFZp686B04100; |
Gene ID : | 6772 |
mRNA Refseq : | NM_007315 |
Protein Refseq : | NP_009330 |
MIM : | 600555 |
UniProt ID : | P42224 |
Chromosome Location : | 2q32.2-q32.3 |
Pathway : | Adipogenesis, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; |
Function : | RNA polymerase II core promoter sequence-specific DNA binding; RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; calcium ion binding; double-stranded DNA binding; enzyme binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; NOT sequence-specific DNA binding transcription factor activity; signal transducer activity; tumor necrosis factor receptor binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionSTAT1 is a signal transduction and transcriptional activator involved in regulating a variety of biological processes such as cell growth, differentiation, apoptosis, and immune response.
Mutations in the STAT1 gene have been found to be associated with a variety of diseases, including autoimmune diseases, primary immunodeficiency diseases, and malignant tumors.
The STAT1 protein is primarily activated by the JAK-STAT signaling pathway. When activated by the receptor, JAK kinase phosphorylates the STAT1 protein, allowing it to form dimers and enter the nucleus to regulate transcription of related genes.
The STAT1 protein plays an important role in many diseases, including tumors, immune-related diseases (such as immunodeficiency diseases, autoimmune diseases), infectious diseases (such as viral infections), etc.
Some activators or inhibitors of the STAT1 protein have been identified. These compounds can be used to study the function of the STAT1 protein and the development of disease treatments.
The STAT1 protein plays an important role in viral infection. It mediates interferon signaling, promotes antiviral immune responses, and inhibits viral replication and transmission.
Customer Reviews (3)
Write a reviewIt is well suited for our laboratory to induce specific immune responses.
It has great application potential and performs well in all aspects.
This protein has high yield and high purity, which greatly improves our production efficiency.
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