Recombinant Human PDE4B cell lysate

PRODUCTS

Home / Products / Cell & Tissue Lysates / Over-Expression Lysates / Recombinant Human PDE4B cell lysate

Recombinant Human PDE4B cell lysate

PDE4B Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : PDE4B-621HCL
Product Overview : Human PDE4B / DPDE4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PDE4B phosphodiesterase 4B, cAMP-specific [ Homo sapiens ]
Official Symbol : PDE4B
Synonyms : PDE4B; phosphodiesterase 4B, cAMP-specific; DPDE4, phosphodiesterase 4B, cAMP specific (dunce (Drosophila) homolog phosphodiesterase E4) , phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila); cAMP-specific 3,5-cyclic phosphodiesterase 4B; phosphodiesterase E4 dunce homolog (Drosophila); PDE32; dunce-like phosphodiesterase E4; cAMP-specific phosphodiesterase-4 B isoform; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); DPDE4; PDE4B5; PDEIVB; MGC126529; DKFZp686F2182;
Gene ID : 5142
mRNA Refseq : NM_001037339
Protein Refseq : NP_001032416
MIM : 600127
UniProt ID : Q07343
Chromosome Location : 1p31
Pathway : DARPP-32 events, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem;
Function : 3,5-cyclic-AMP phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry


  • Note: There will be extra charge for optional service!
  • Verification Code Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy