Recombinant Human PADI4 cell lysate
Cat.No. : | PADI4-630HCL |
Product Overview : | Human PADI4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PADI4 peptidyl arginine deiminase, type IV [ Homo sapiens ] |
Official Symbol : | PADI4 |
Synonyms : | PADI4; peptidyl arginine deiminase, type IV; PADI5, peptidyl arginine deiminase, type V; protein-arginine deiminase type-4; PAD; PDI4; PDI5; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; PAD4; PADI5; |
Gene ID : | 23569 |
mRNA Refseq : | NM_012387 |
Protein Refseq : | NP_036519 |
MIM : | 605347 |
UniProt ID : | Q9UM07 |
Chromosome Location : | 1p36.13 |
Pathway : | protein citrullination, organism-specific biosystem; protein citrullination, conserved biosystem; |
Function : | calcium ion binding; hydrolase activity; protein-arginine deiminase activity; |
Products Types
◆ Recombinant Protein | ||
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PADI4-037H | Recombinant Human PADI4 Protein, His/GST-tagged | +Inquiry |
PADI4-203H | Recombinant Human PADI4 Protein, MYC/DDK-tagged | +Inquiry |
PADI4-0218H | Recombinant Human PADI4 Protein (A2-P663), Tag Free | +Inquiry |
Padi4-038M | Recombinant Mouse Padi4 Protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionPADI4 is predominantly expressed in various tissues, including synovial tissues, immune cells, and certain epithelial cells.
PADI4 plays a crucial role in citrullination, a process that converts arginine residues to citrulline, influencing protein structure and function.
Peptidyl arginine deiminase 4 (PADI4) primarily functions in cellular processes, contributing to the post-translational modification of proteins through citrullination.
In the regulation of immune responses, PADI4 is implicated in autoimmune diseases such as rheumatoid arthritis, where it contributes to autoantibody generation.
Dysregulation or abnormal expression of PADI4 is associated with autoimmune disorders, particularly rheumatoid arthritis.
There are potential therapeutic implications related to targeting PADI4 in autoimmune disorders, making it a focus for drug development.
Specific substrates of PADI4 include histones and other proteins, impacting cellular processes like gene regulation and signaling pathways.
Customer Reviews (3)
Write a reviewThe minimal variation across different batches enhanced the repeatability of our results.
The product's role in promoting collaboration and teamwork positively impacted the quality of our research.
The product's compatibility with various instruments and techniques promoted teamwork in our interdisciplinary projects.
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