Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human PADI4 cell lysate

Cat.No. : PADI4-630HCL
Product Overview : Human PADI4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PADI4 peptidyl arginine deiminase, type IV [ Homo sapiens ]
Official Symbol : PADI4
Synonyms : PADI4; peptidyl arginine deiminase, type IV; PADI5, peptidyl arginine deiminase, type V; protein-arginine deiminase type-4; PAD; PDI4; PDI5; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; PAD4; PADI5;
Gene ID : 23569
mRNA Refseq : NM_012387
Protein Refseq : NP_036519
MIM : 605347
UniProt ID : Q9UM07
Chromosome Location : 1p36.13
Pathway : protein citrullination, organism-specific biosystem; protein citrullination, conserved biosystem;
Function : calcium ion binding; hydrolase activity; protein-arginine deiminase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
In which tissues or cell types is PADI4 predominantly expressed? 09/25/2022

PADI4 is predominantly expressed in various tissues, including synovial tissues, immune cells, and certain epithelial cells.

How does PADI4 contribute to the post-translational modification of proteins, particularly through citrullination? 07/27/2020

PADI4 plays a crucial role in citrullination, a process that converts arginine residues to citrulline, influencing protein structure and function.

What is the primary function of peptidyl arginine deiminase 4 (PADI4) in cellular processes? 06/16/2020

Peptidyl arginine deiminase 4 (PADI4) primarily functions in cellular processes, contributing to the post-translational modification of proteins through citrullination.

What is the role of PADI4 in the regulation of immune responses and autoimmune diseases? 05/25/2020

In the regulation of immune responses, PADI4 is implicated in autoimmune diseases such as rheumatoid arthritis, where it contributes to autoantibody generation.

How is dysregulation or abnormal expression of PADI4 implicated in various diseases, including rheumatoid arthritis? 12/31/2019

Dysregulation or abnormal expression of PADI4 is associated with autoimmune disorders, particularly rheumatoid arthritis.

Are there therapeutic implications or potential targets related to PADI4 in the context of autoimmune disorders? 08/26/2018

There are potential therapeutic implications related to targeting PADI4 in autoimmune disorders, making it a focus for drug development.

Are there specific substrates of PADI4, and what cellular processes are affected by its citrullination activity? 07/14/2016

Specific substrates of PADI4 include histones and other proteins, impacting cellular processes like gene regulation and signaling pathways.

Customer Reviews (3)

Write a review
Reviews
12/05/2020

    The minimal variation across different batches enhanced the repeatability of our results.

    06/14/2018

      The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

      01/23/2017

        The product's compatibility with various instruments and techniques promoted teamwork in our interdisciplinary projects.

        Ask a Question for All PADI4 Products

        Required fields are marked with *

        My Review for All PADI4 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends