Recombinant Human ABHD4 cell lysate
Cat.No. : | ABHD4-675HCL |
Product Overview : | Human ABHD4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | ABHD4 abhydrolase domain containing 4 [ Homo sapiens ] |
Official Symbol : | ABHD4 |
Synonyms : | ABHD4; abhydrolase domain containing 4; abhydrolase domain-containing protein 4; FLJ12816; alpha/beta-hydrolase 4; lyso-N-acylphosphatidylethanolamine lipase; ABH4; |
Gene ID : | 63874 |
mRNA Refseq : | NM_022060 |
Protein Refseq : | NP_071343 |
UniProt ID : | Q8TB40 |
Chromosome Location : | 14q11.1 |
Function : | hydrolase activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionSure, we offer MSDS files and data sheets with detailed product information.
We can offer a bulk quote for each product available on our website. For specific prices, please contact our sales representatives.
The GST tag promotes protein solubility and is used for affinity purification, it may or may not affect protein activity. If you are concerned that the tag may interfere with protein activity, Creative BioMart can also provide custom tag-free ABHD4 protein service.
Sure, and we recommend that you select the conserved motif of the ABHD4 enzyme of the corresponding species.
For example, amino acid residues 50–69 of the mouse ABHD4 protein.
Please provide our scientists with detailed information about the ABHD4 protein you are going to use to prepare the primary antibody, including species, amino acid residue sequence and fusion tag. Experts will offer specific solutions according to experimental requirements.
Customer Reviews (2)
Write a reviewI am glad that I chose ABHD4 recombinant protein from Creative BioMart, it saved me a lot of time.
The scientists at Creative BioMart customized the suitable recombinant protein for me, and it was a very satisfying collaboration.
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