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Recombinant Mouse KLK1 cell lysate

Cat.No. : KLK1-743MCL
Product Overview : Mouse KLK1 / Kallikrein 1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Klk1 kallikrein 1 [ Mus musculus ]
Official Symbol : KLK1
Synonyms : KLK1; kallikrein 1; kallikrein-1; KAL-B; kallikrein 6; renal kallikrein; tissue kallikrein-6; true tissue kallikrein; glandular kallikrein K1; kallikrein renal/pancreas/salivary; Kal; Klk6; mGk-6; Klk1b6; 0610007D04Rik;
Gene ID : 16612
mRNA Refseq : NM_010639
Protein Refseq : NP_034769
Pathway : Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem;
Function : catalytic activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is KLK1 involved in coagulation and fibrinolysis? 08/10/2022

KLK1 participates in the regulation of blood clotting and fibrinolysis by influencing the activation of certain coagulation factors and promoting fibrinolysis.

How does KLK1 impact renal function? 06/06/2020

KLK1 contributes to renal function by promoting vasodilation in the renal blood vessels and influencing sodium and water excretion, which can be beneficial in kidney diseases.

Is KLK1 associated with cancer? 03/26/2020

Some studies suggest that KLK1 may be involved in cancer progression, but the relationship is complex and not fully understood. Further research is needed.

Are there any clinical applications of KLK1 in inflammatory conditions? 05/18/2019

Some studies suggest that KLK1-based therapies may have potential in managing inflammatory conditions, but more research is needed for conclusive evidence.

Are there any KLK1-based therapies for cancer? 02/21/2016

The potential use of KLK1 in cancer therapy is an area of investigation, but no specific KLK1-based cancer therapies have been established yet.

Customer Reviews (3)

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Reviews
04/24/2019

    Whether measuring analyte concentrations, screening for specific interactions, or monitoring immune responses, this protein's exceptional performance in ELISA experiments is truly commendable.

    06/30/2018

      Its high quality, reliability, and exceptional performance make it an excellent choice for a wide range of experimental applications.

      12/12/2017

        This strong track record of excellence further attests to the KLK1 protein's capability to meet the rigorous demands of research projects.

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