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Recombinant Rat CDH13 cell lysate

Cat.No. : CDH13-802RCL
Product Overview : Rat CDH13 / Cadherin-13 / H Cadherin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cdh13 cadherin 13 [ Rattus norvegicus ]
Official Symbol : CDH13
Synonyms : CDH13; cadherin 13; cadherin-13; Cdht; Tcad; T-cadherin; MGC93172;
Gene ID : 192248
mRNA Refseq : NM_138889
Protein Refseq : NP_620244
Pathway : Adherens junctions interactions, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem;
Function : adiponectin binding; cadherin binding; lipoprotein particle binding; low-density lipoprotein particle binding; molecular_function; protein homodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are possible future directions for CDH13 research? 11/27/2022

Future research on CDH13 may involve more in-depth research on molecular mechanisms, new drug development, and targeted therapeutic strategies based on CDH13.

How about the interactions between CDH13 and other molecules or drugs? 10/12/2022

There are multiple interactions between CDH13 and other molecules and drugs, including growth factors, cytokines, and drugs.

Are there population differences in CDH13 treatment? 11/26/2021

It is unclear whether there is a population difference in the treatment of CDH13. However, due to factors such as individual differences and genetic polymorphisms, different populations may respond differently to treatment.

Can the expression level of CDH13 be used as a biomarker for disease diagnosis or prognosis? 11/03/2021

At present, the expression level of CDH13 as a biomarker for disease diagnosis or prognosis is not sufficient, and further validation is needed.

What is the role of CDH13 in cardiovascular disease? 02/26/2020

Studies have shown that CDH13 is associated with the occurrence and progression of cardiovascular disease, and it can affect the production of blood vessels, the development of heart cells, and heart function.

Is CDH13 related to the occurrence and development of tumors? 12/05/2019

Recent studies have shown that CDH13 is expressed abnormally in some tumors, which may be related to the occurrence and progression of tumors.

Customer Reviews (3)

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Reviews
02/17/2023

    It is highly active, and a small amount can play a role, saving reagents.

    07/12/2022

      CDH13 is easy to store and can be stored for a long time, which is very suitable for our research needs.

      09/14/2021

        No significant differences were found between different batches, and the batch-to-batch reproducibility was good.

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