Recombinant Canine GFRA1 cell lysate
Cat.No. : | GFRA1-966CCL |
Product Overview : | Canine GFRA1 / GFR alpha-1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Canine |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | GFRA1 GDNF family receptor alpha 1 [ Canis lupus familiaris ] |
Official Symbol : | GFRA1 |
Gene ID : | 609114 |
mRNA Refseq : | XM_005637762 |
Protein Refseq : | XP_005637819 |
Chromosome Location : | chromosome: 28 |
Pathway : | Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem |
Products Types
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GFRA1-2170R | Recombinant Rat GFRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
GFRA1-2790H | Recombinant Human GFRA1 protein(51-280 aa), N-SUMO & C-His-tagged | +Inquiry |
◆ Lysates | ||
GFRA1-2692MCL | Recombinant Mouse GFRA1 cell lysate | +Inquiry |
GFRA1-961RCL | Recombinant Rat GFRA1 cell lysate | +Inquiry |
GFRA1-2426HCL | Recombinant Human GFRA1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, several clinical trials are exploring the modulation of GFRA1 for the treatment of neurodegenerative diseases.
GFRA1 is involved in neuroprotection by promoting the survival and maintenance of neurons, making it a potential target for therapeutic interventions.
GFRA1 is crucial for the development and function of the nervous system as it participates in the regulation of neuronal survival and differentiation.
Some therapeutic strategies aim to harness GFRA1 for promoting neuroregeneration, especially in the context of nerve injuries.
Research suggests that alterations in GFRA1 expression may serve as a potential biomarker for specific neurological disorders.
Customer Reviews (3)
Write a reviewWith their expertise, I can navigate through experimental hurdles, optimize protocols, and ensure the seamless integration of the GFRA1 protein into my investigations.
The GFRA1 protein showcases unparalleled quality, ensuring it surpasses expectations and satisfactorily addresses my experimental requirements.
Their knowledgeable and responsive team stands ready to address any concerns or challenges I may encounter.
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