Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Canine GFRA1 cell lysate

Cat.No. : GFRA1-966CCL
Product Overview : Canine GFRA1 / GFR alpha-1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Canine
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : GFRA1 GDNF family receptor alpha 1 [ Canis lupus familiaris ]
Official Symbol : GFRA1
Gene ID : 609114
mRNA Refseq : XM_005637762
Protein Refseq : XP_005637819
Chromosome Location : chromosome: 28
Pathway : Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any ongoing clinical trials targeting GFRA1 for neurodegenerative diseases? 05/25/2021

Yes, several clinical trials are exploring the modulation of GFRA1 for the treatment of neurodegenerative diseases.

How does GFRA1 contribute to neuroprotection? 04/25/2020

GFRA1 is involved in neuroprotection by promoting the survival and maintenance of neurons, making it a potential target for therapeutic interventions.

How does GFRA1 influence the development and function of the nervous system? 10/10/2017

GFRA1 is crucial for the development and function of the nervous system as it participates in the regulation of neuronal survival and differentiation.

Are there any therapeutic strategies targeting GFRA1 for neuroregeneration? 10/26/2016

Some therapeutic strategies aim to harness GFRA1 for promoting neuroregeneration, especially in the context of nerve injuries.

Can GFRA1 protein be a biomarker for certain neurological disorders? 07/12/2016

Research suggests that alterations in GFRA1 expression may serve as a potential biomarker for specific neurological disorders.

Customer Reviews (3)

Write a review
Reviews
03/24/2022

    With their expertise, I can navigate through experimental hurdles, optimize protocols, and ensure the seamless integration of the GFRA1 protein into my investigations.

    08/19/2020

      The GFRA1 protein showcases unparalleled quality, ensuring it surpasses expectations and satisfactorily addresses my experimental requirements.

      03/11/2020

        Their knowledgeable and responsive team stands ready to address any concerns or challenges I may encounter.

        Ask a Question for All GFRA1 Products

        Required fields are marked with *

        My Review for All GFRA1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends