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Recombinant Human FUCA1 cell lysate

Cat.No. : FUCA1-1212HCL
Product Overview : Human FUCA1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FUCA1 fucosidase, alpha-L- 1, tissue [ Homo sapiens ]
Official Symbol : FUCA1
Synonyms : FUCA1; fucosidase, alpha-L- 1, tissue; tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1; FUCA;
Gene ID : 2517
mRNA Refseq : NM_000147
Protein Refseq : NP_000138
MIM : 612280
UniProt ID : P04066
Chromosome Location : 1p34
Pathway : Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem;
Function : alpha-L-fucosidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do genetic variations in FUCA1 affect its enzymatic activity? 12/10/2022

Genetic mutations in FUCA1 can lead to deficient enzyme activity, affecting glycan metabolism.

What is the impact of altered FUCA1 activity on lysosomal storage diseases? 02/27/2022

Altered FUCA1 activity is associated with lysosomal storage diseases, such as fucosidosis, due to the accumulation of undigested glycans.

What is the primary role of FUCA1 in cellular metabolism? 02/28/2021

FUCA1, an alpha-L-fucosidase, is involved in the breakdown of complex sugars (glycans) in cellular metabolism.

What role does FUCA1 play in lysosomal function? 10/22/2020

FUCA1 is crucial for normal lysosomal function, aiding in the breakdown of glycoproteins and glycolipids.

How does FUCA1 contribute to glycan breakdown? 08/26/2018

It helps in glycan degradation by cleaving fucose residues, a key step in glycan processing.

How does FUCA1 interact with other enzymes in glycan metabolism? 06/27/2018

FUCA1 works alongside other enzymes in glycan metabolism, ensuring efficient breakdown and recycling of cellular components.

What potential therapeutic applications arise from targeting FUCA1 in metabolic disorders? 06/05/2017

Targeting FUCA1 could offer therapeutic strategies for treating lysosomal storage disorders and related metabolic conditions.

Customer Reviews (3)

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Reviews
08/16/2021

    Precise results, a trusted partner in our experiments.

    05/11/2020

      Dependable service, vital for accurate protein analysis.

      09/12/2019

        Efficient support, accelerates our research progress.

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