Recombinant Human FUCA1 cell lysate
Cat.No. : | FUCA1-1212HCL |
Product Overview : | Human FUCA1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | FUCA1 fucosidase, alpha-L- 1, tissue [ Homo sapiens ] |
Official Symbol : | FUCA1 |
Synonyms : | FUCA1; fucosidase, alpha-L- 1, tissue; tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1; FUCA; |
Gene ID : | 2517 |
mRNA Refseq : | NM_000147 |
Protein Refseq : | NP_000138 |
MIM : | 612280 |
UniProt ID : | P04066 |
Chromosome Location : | 1p34 |
Pathway : | Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem; |
Function : | alpha-L-fucosidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; sugar binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionGenetic mutations in FUCA1 can lead to deficient enzyme activity, affecting glycan metabolism.
Altered FUCA1 activity is associated with lysosomal storage diseases, such as fucosidosis, due to the accumulation of undigested glycans.
FUCA1, an alpha-L-fucosidase, is involved in the breakdown of complex sugars (glycans) in cellular metabolism.
FUCA1 is crucial for normal lysosomal function, aiding in the breakdown of glycoproteins and glycolipids.
It helps in glycan degradation by cleaving fucose residues, a key step in glycan processing.
FUCA1 works alongside other enzymes in glycan metabolism, ensuring efficient breakdown and recycling of cellular components.
Targeting FUCA1 could offer therapeutic strategies for treating lysosomal storage disorders and related metabolic conditions.
Customer Reviews (3)
Write a reviewPrecise results, a trusted partner in our experiments.
Dependable service, vital for accurate protein analysis.
Efficient support, accelerates our research progress.
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