Recombinant Mouse PRLR cell lysate
Cat.No. : | PRLR-1451MCL |
Product Overview : | Mouse PRLR / Prolactin receptor derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Prlr prolactin receptor [ Mus musculus ] |
Official Symbol : | PRLR |
Synonyms : | PRLR; prolactin receptor; PRL-R; prolactin receptor related sequence 1; Pr-1; Pr-3; AI987712; Prlr-rs1; |
Gene ID : | 19116 |
mRNA Refseq : | NM_001253781 |
Protein Refseq : | NP_001240710 |
Pathway : | Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; |
Function : | cytokine receptor activity; metal ion binding; peptide hormone binding; prolactin receptor activity; prolactin receptor activity; prolactin receptor activity; protein binding; protein kinase binding; receptor activity; |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionPRLR overexpression or dysregulation has been associated with breast cancer. It can promote cell proliferation and survival through aberrant signaling pathways.
Tissue-specific factors and the presence of co-receptors contribute to the diversity of PRLR signaling outcomes in different tissues and cell types.
The JAK-STAT signaling pathway is a primary pathway activated by PRLR. It involves the activation of Janus kinases (JAKs) and signal transducers and activators of transcription (STATs).
PRLR undergoes endocytosis upon ligand binding, and the internalized receptor-ligand complex is trafficked through intracellular compartments, influencing downstream signaling and receptor recycling.
Phosphorylation is a crucial post-translational modification of PRLR, affecting its activation and downstream signaling. Other modifications, such as glycosylation, also play roles.
Customer Reviews (3)
Write a reviewConsistent quality shines through—this protein has become indispensable.
This protein streamlines our experiments.
Fast shipping made this purchase convenient for our lab operations.
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