Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human FAM3B cell lysate

Cat.No. : FAM3B-1648HCL
Product Overview : Human FAM3B derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FAM3B family with sequence similarity 3, member B [ Homo sapiens ]
Official Symbol : FAM3B
Synonyms : FAM3B; family with sequence similarity 3, member B; C21orf11, chromosome 21 open reading frame 11; protein FAM3B; 2 21; C21orf76; D21M16SJHU19e; ORF9; PRED44; pancreatic derived factor; pancreatic-derived factor; cytokine-like protein 2-21; 2-21; PANDER; C21orf11;
Gene ID : 54097
mRNA Refseq : NM_058186
Protein Refseq : NP_478066
MIM : 608617
UniProt ID : P58499
Chromosome Location : 21q22.3
Function : cytokine activity;

Products Types

◆ Recombinant Protein
FAM3B-358H Recombinant Human FAM3B Protein, MYC/DDK-tagged +Inquiry
Fam3b-2938M Recombinant Mouse Fam3b Protein, Myc/DDK-tagged +Inquiry
FAM3B-3752H Recombinant Human FAM3B Protein, GST-tagged +Inquiry
FAM3B-750H Recombinant Human FAM3B Protein, Fc-tagged +Inquiry
FAM3B-28821TH Recombinant Human FAM3B, His-tagged +Inquiry

See All FAM3B Recombinant Protein

Related Gene

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
What role does FAM3B protein play in tumors? 12/05/2022

Studies have shown that FAM3B protein may play an oncogene role in tumors and promote tumor initiation and progression.

Can FAM3B protein be used as a biomarker? 06/19/2021

Since FAM3B protein plays an important role in a variety of diseases and physiological processes, it may be used as a biomarker to monitor the onset and progression of diseases.

In which biological processes does FAM3B protein play a role? 08/13/2020

FAM3B protein plays an important role in the process of cell proliferation, differentiation, migration, etc., and is also related to physiological processes such as insulin secretion and glucose metabolism.

How to use FAM3B protein for drug design and development? 07/02/2020

Since FAM3B protein plays an important role in a variety of diseases, it may become a target for drug design and development, and specific drug design and development need to combine methods such as computer-aided design and experimental validation.

How does FAM3B protein interact with other proteins or molecules? 05/12/2020

FAM3B protein fulfills its function by binding to ligands or interacting with other proteins, which may involve signal transduction pathways, cytoskeletal structure, etc.

What is the structure-function relationship of FAM3B protein? 03/04/2020

The structure of FAM3B protein determines its function, specifically its N-terminal domain may involve binding to ligands, while its C-terminal domain involves interactions with other proteins.

Customer Reviews (3)

Write a review
Reviews
03/26/2022

    Compared to other brands I've used, this one is more stable and reliable.

    03/04/2022

      The preparation process of FAM3B is very simple and can be easily prepared.

      08/18/2021

        The detection method is very sensitive and can detect trace amounts of protein.

        Ask a Question for All FAM3B Products

        Required fields are marked with *

        My Review for All FAM3B Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends