Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at BIO-Europe Spring|March 18–20, 2024|Booth #34

Recombinant Human FAP cell lysate

Cat.No. : FAP-1881HCL
Product Overview : Human FAP / Seprase derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FAP fibroblast activation protein, alpha [ Homo sapiens ]
Official Symbol : FAP
Synonyms : FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158;
Gene ID : 2191
mRNA Refseq : NM_004460
Protein Refseq : NP_004451
MIM : 600403
UniProt ID : Q12884
Chromosome Location : 2q23
Function : dipeptidyl-peptidase activity; endopeptidase activity; metalloendopeptidase activity; peptidase activity; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; serine-type endopeptidase activity; serine-type peptidase activity; contributes_to serine-type peptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
How does FAP contribute to immunosuppression in the tumor microenvironment? 04/18/2023

FAP can suppress the immune response by modulating the activity of certain immune cells, creating an immunosuppressive tumor microenvironment.

How can FAP expression be used as a prognostic marker in cancer patients? 02/03/2023

High levels of FAP expression in tumors have been associated with poor prognosis, making it a potential prognostic marker for certain cancers.

Are there ongoing clinical trials exploring FAP-targeted therapies? 05/04/2021

Yes, several clinical trials are investigating the safety and efficacy of FAP-targeted therapies in various cancer types.

Can FAP be utilized as a therapeutic target beyond cancer treatment? 11/19/2020

FAP-targeted therapies are being explored for conditions such as fibrotic diseases and rheumatoid arthritis, extending its potential clinical applications.

How is imaging technology utilized to detect FAP expression in tumors? 09/17/2018

Molecular imaging techniques, such as positron emission tomography (PET), can be employed to visualize FAP expression in tumors noninvasively.

Customer Reviews (3)

Write a review
Reviews
08/11/2020

    It enables accurate detection and quantification of FAP protein levels in samples, providing crucial insights into its expression, regulation, and potential functional changes under different experimental conditions.

    05/04/2020

      FAP protein is highly recommended for researchers due to its excellent performance in various assays, particularly in Western blotting (WB) experiments, and its applicability in protein electron microscopy structure analysis.

      01/02/2019

        FAP protein is highly recommended for researchers, as it demonstrates excellent performance in WB experiments and has proven useful in protein electron microscopy structure analysis.

        Ask a Question for All FAP Products

        Required fields are marked with *

        My Review for All FAP Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends