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Recombinant Human IL23 cell lysate

Cat.No. : IL23-1893HCL
Product Overview : Human Human IL-23 / Interleukin-23 ( IL-23A & IL-12B Heterodimer ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ]
Official Symbol : IL23A
Synonyms : P19; SGRF; IL-23; IL-23A; IL23P19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
Gene ID : 51561
mRNA Refseq : NM_016584
Protein Refseq : NP_057668
MIM : 605580
UniProt ID : Q9NPF7
Chromosome Location : 12q13.3
Pathway : ATF-2 transcription factor network, organism-specific biosystem; Inflammatory bowel disease (IBD), conserved biosystem
Function : cytokine activity; contributes_to interleukin-23 receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Does IL23 expression correlate with disease severity in psoriasis? 05/12/2022

IL23 expression correlates with disease severity in psoriasis.

Does IL23 expression vary between different immune cell types? 06/03/2021

IL23 expression varies among dendritic cells and macrophages.

What are the downstream targets of IL23 signaling? 07/24/2020

IL23 activates STAT3 to modulate downstream targets.

Does IL23 interact with other cytokines in a signaling cascade? 06/07/2019

IL23 interacts with IL-12 to form a signaling cascade.

Is IL23 involved in the activation of STAT3? 04/29/2019

IL23 is involved in the activation of STAT3.

How does IL23 expression change in autoimmune diseases? 10/02/2018

IL23 expression is upregulated in autoimmune diseases like Crohn's.

What is the half-life of IL23 in plasma? 01/14/2018

The half-life of IL23 in plasma is approximately 2 hours.

How does IL23 affect macrophage polarization? 10/25/2017

IL23 induces M1 macrophage polarization.

What is the role of IL23 in Th17 cell differentiation? 03/19/2017

IL23 promotes Th17 cell differentiation by activating STAT3.

How does IL23 contribute to tissue inflammation? 01/27/2016

IL23 contributes to tissue inflammation by activating macrophages.

Customer Reviews (3)

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Reviews
12/17/2021

    High purity levels in CHIP

    12/26/2018

      Effective in T-cell differentiation.

      09/25/2018

        Suitable for signaling studies.

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