Active Recombinant Human IL23A&IL12B Protein, His-tagged, Low Endotoxin

• Cat.No.: IL23A&IL12B-209H
• Product Overview: Active Recombinant Human IL23A&IL12B Protein(NP_002178.2&NP_057668.1)(Met1-Ser328&Arg20-Pro189) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-328 aa&20-189 aa
• Form: Supplied as sterile PBS, pH 7.4.
• Bio-activity: 1.Refer to: Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.1-1ng/mL. 2.Refer to: Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag), the EC50 is 15-60 ng/mL . 3.Refer to: Immobilized Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag), the EC50 is 25-80 ng/mL . 4.Refer to: Immobilized Anti-IL-12 & IL-23 (P40)(Research Grade Ustekin*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL12B & IL23A Heterodimer) Protein (His Tag), the EC50 is 6-18 ng/mL . 5.Refer to: Recombinant Human IL-23 Receptor Protein, hFc Tag captured on Protein A chip can bind Recombinant Human IL-23 Protein, His Tag with an affinity constant of 0.6961 nM as determined in an SPR assay. 6.Refer to: Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag with an affinity constant of 1.25 nM as determined in BLI assay.
• Molecular Mass: The recombinant Human IL23(IL23A&IL12B) consists of 502 amino acids and predicts a molecular mass of 55.8 kDa. The band migrates approximately 50-60 kDa in the SDS-PAGE under reducing conditions.
• Endotoxin: < 0.5 EU per mg protein.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ]
• Official Symbol: Il23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL 23; IL 23A; IL23P19; interleukin six; G CSF related factor; P19; SGRF; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; IL-23; IL-23A; MGC79388
• Gene ID: 51561
• mRNA Refseq: NM_016584
• Protein Refseq: NP_057668
• MIM: 605580
• UniProt ID: Q9NPF7
• Gene Name 2: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol 2: Il12B
• Synonyms 2: IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B
• Gene ID 2: 3593
• mRNA Refseq 2: NM_002187
• Protein Refseq 2: NP_002178
• MIM 2: 161561
• UniProt ID 2: P29460