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Recombinant Human TREM1 cell lysate

Cat.No. : TREM1-2140HCL
Product Overview : Human TREM1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TREM1 triggering receptor expressed on myeloid cells 1 [ Homo sapiens ]
Official Symbol : TREM1
Synonyms : TREM1; triggering receptor expressed on myeloid cells 1; CD354; TREM 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1; TREM-1;
Gene ID : 54210
mRNA Refseq : NM_001242589
Protein Refseq : NP_001229518
MIM : 605085
UniProt ID : Q9NP99
Chromosome Location : 6p21.1
Pathway : Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem;
Function : receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does TREM1 protein have antimicrobial effects? 11/03/2019

TREM1 protein can enhance inflammatory response during infection, but its specific relationship with antibacterial effect still needs further study.

What is the role of TREM1 protein in the treatment of lung diseases? 08/25/2019

High expression of the TREM1 protein in lung disease can trigger an excessive inflammatory response, and controlling its activity may help reduce the symptoms and inflammatory damage of lung disease.

How is the TREM1 protein related to bacterial infections? 04/22/2019

When bacterial infection occurs, the expression of the TREM1 protein increases, which enhances the inflammatory response, which helps clear the infection and regulate the inflammatory process.

What immune response is the TREM1 protein involved in? 03/20/2019

TREM1 protein is involved in immune response processes such as macrophage activation, release of inflammatory mediators, and recruitment and regulation of immune cells.

What are the implications of the study of TREM1 protein for the treatment of inflammatory diseases? 02/04/2019

The study of TREM1 protein helps to understand the regulatory mechanism of inflammatory response and provide new targets and strategies for the treatment of inflammatory diseases.

Does the TREM1 protein have the potential to predict and monitor disease progression? 01/12/2019

High expression of the TREM1 protein is associated with the development and prognosis of certain diseases and can therefore serve as a biomarker for predicting and monitoring disease progression.

Customer Reviews (3)

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Reviews
05/19/2022

    The exceptional stability of TREM1 makes it an invaluable tool for a wide range of applications.

    01/18/2020

      Manufacturer's commitment to environmental sustainability is evident in its production processes, using green and renewable resources.

      10/06/2019

        TREM1 has a short half-life, making it more effective and less likely to cause side effects compared to other similar proteins.

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