Recombinant Human CFH cell lysate
Cat.No. : | CFH-2409HCL |
Product Overview : | Human CFH / Complement Factor H derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CFH complement factor H [ Homo sapiens ] |
Official Symbol : | CFH |
Synonyms : | CFH; complement factor H; H factor 1 (complement) , HF, HF1, HF2; age related maculopathy susceptibility 1; ARMS1; beta 1H; FHL1; H factor 2 (complement); HUS; beta-1H; factor H; factor H-like 1; beta-1-H-globulin; H factor 1 (complement); adrenomedullin binding protein; complement factor H, isoform b; age-related maculopathy susceptibility 1; FH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; CFHL3; MGC88246; |
Gene ID : | 3075 |
mRNA Refseq : | NM_000186 |
Protein Refseq : | NP_000177 |
MIM : | 134370 |
UniProt ID : | P08603 |
Chromosome Location : | 1q32 |
Pathway : | Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Regulation of Complement cascade, organism-specific biosystem; Staphylococcus aureus infection, organism-specific biosystem; |
Products Types
◆ Recombinant Protein | ||
Cfh-761M | Recombinant Mouse Cfh Protein, His-tagged | +Inquiry |
Cfh-1612M | Recombinant Mouse Cfh Protein, His (Fc)-Avi-tagged | +Inquiry |
CFH-762H | Recombinant Human CFH Protein, His-tagged | +Inquiry |
CFH-763H | Recombinant Human CFH Protein, His-tagged | +Inquiry |
CFH-1168H | Recombinant Human CFH Protein, GST-Tagged | +Inquiry |
◆ Native Protein | ||
CFH-23H | Active Native Human Complement factor H | +Inquiry |
CFH-115H | Active Native Human Factor H | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMutations in CFH increase the risk of aHUS, affecting complement regulation and leading to kidney damage.
CFH controls the complement system, preventing its overactivation and protecting against immune damage.
CFH modulates the immune response to bacterial infections, balancing defense mechanisms.
CFH deficiency is linked to AMD, due to impaired regulation of the complement pathway in the retina.
CFH shields host cells from complement-mediated injury, maintaining tissue integrity.
CFH regulates inflammation and maintains immune balance, crucial for preventing excessive immune responses.
Changes in CFH function can impact kidney health, potentially leading to renal diseases due to altered complement activity.
Customer Reviews (3)
Write a reviewExceptional protein expression optimization support, highly appreciated assistance.
Precise protein quantification, ensures data accuracy in our research.
Timely antibody validation, an invaluable asset for our laboratory.
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