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Recombinant Mouse ACP5 cell lysate

Cat.No. : ACP5-2431MCL
Product Overview : Mouse ACP5 / TRAP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Acp5 acid phosphatase 5, tartrate resistant [ Mus musculus ]
Official Symbol : ACP5
Synonyms : ACP5; acid phosphatase 5, tartrate resistant; tartrate-resistant acid phosphatase type 5; TR-AP; trATPase; acid phosphatase type V; type 5 acid phosphatase; tartrate-resistant acid ATPase; TRAP; TRACP;
Gene ID : 11433
mRNA Refseq : NM_001102404
Protein Refseq : NP_001095874
Pathway : Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; NAD phosphorylation and dephosphorylation, organism-specific biosystem; NAD salvage pathway II, organism-specific biosystem;
Function : acid phosphatase activity; acid phosphatase activity; acid phosphatase activity; ferric iron binding; ferrous iron binding; hydrolase activity; metal ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What will happen if loss ACP5 in vivo? 02/19/2022

the loss of Acp5 significantly protected mice from BLM-induced lung injury and fibrosis, accompanied by a marked reduction in myofibroblast accumulation.

Could please list three drugs for ACP5 gene? 09/26/2021

Alteplase, Filgrastim, and Streptozocin.

What are four reactome pathways for ACP5 gene? 06/11/2021

They are Metabolism; Metabolism of vitamins and cofactors; Metabolism of water-soluble vitamins and cofactors; Vitamin B2 (riboflavin) metabolism

How does ACP5 epithelial-mesenchymal transition (EMT)? 01/31/2021

ACP5 promoted A549 and NCI-H1975 cells undergoing epithelial-mesenchymal transition (EMT) via P53/Smad3 signaling.

Can you briefly descibe the molecular function of ACP5 protein? 05/22/2020

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

How about the human health condition related to ACP5 gene changes? 05/20/2020

Diseases associated with ACP5 include spondyloenchondrodysplasia with Immune dysregulation and Hairy Cell Leukemia. Among its related pathways are metabolism of water-soluble. vitamins and cofactors and Metabolism

What is the effect of ACP5 serum concentration on Idiopathic pulmonary fibrosis (IPF) patients? 05/15/2019

Serum concentration of ACP5 was likely associated with decreased lung function in IPF patients.

What is the role of ACP5 on the process of fibroblast's cell differentiation and proliferation? 03/30/2019

Acp5 is upregulated by transforming growth factor-β1 (TGF-β1) in a TGF-β receptor 1 (TGFβR1)/Smad family member 3 (Smad3)-dependent manner, after which Acp5 dephosphorylates p-β-catenin at serine 33 and threonine 41, inhibiting the degradation of β-catenin and subsequently enhancing β-catenin signaling in the nucleus, which promotes the differentiation, proliferation and migration of fibroblast.

What functions does ACP5 could apply when we act it as biomarks? 08/03/2018

ACP5 is evaluated as osteoclast differentiation/activity markers in serum and in culture.

What will happen to mice if ACP5 deficiency present? 04/08/2018

Acp5 deficiency protects mice from BLM-induced lung injury and fibrosis coupled with a significant reduction of fibroblast differentiation and proliferation.

Customer Reviews (5)

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Reviews
08/03/2022

    Arrived prompt - good service

    09/11/2021

      Much better than similar products that I've used.

      07/02/2021

        Used in protein electron microscopy structure analysis.Great.

        01/10/2021

          Great performance in WB :)

          06/20/2018

            Patient customer service, love it.

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