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Recombinant Human REG3A cell lysate

Cat.No. : REG3A-2496HCL
Product Overview : Human REG3A / HIP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : REG3A regenerating islet-derived 3 alpha [ Homo sapiens ]
Official Symbol : REG3A
Synonyms : REG3A; regenerating islet-derived 3 alpha; pancreatitis associated protein , PAP; regenerating islet-derived protein 3-alpha; HIP; PAP1; PBCGF; REG III; REG3; REG-3-alpha; reg III-alpha; PAP homologous protein; human proislet peptide; pancreatitis-associated protein 1; proliferation-inducing protein 34; proliferation-inducing protein 42; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; regenerating islet-derived protein III-alpha; PAP; PAP-H; REG-III; FLJ18565;
Gene ID : 5068
mRNA Refseq : NM_002580
Protein Refseq : NP_002571
MIM : 167805
UniProt ID : Q06141
Chromosome Location : 2p12
Function : binding; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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In what gastrointestinal disorders is REG3A protein implicated? 02/04/2023

REG3A has been associated with inflammatory bowel diseases (IBD), such as Crohn's disease and ulcerative colitis, where its dysregulation can contribute to inflammation and tissue damage.

In what way does REG3A impact the microbiota of the gastrointestinal tract? 08/12/2021

REG3A plays a role in maintaining the balance of the gut microbiota by influencing the composition of the microbial community.

Can REG3A be used as a biomarker for certain diseases? 07/28/2021

Yes, altered levels of REG3A have been observed in several diseases, making it a potential biomarker for gastrointestinal disorders and certain cancers.

How does REG3A function in the context of the immune response? 01/15/2021

REG3A has immunomodulatory effects, participating in the regulation of the immune response, particularly in the defense against bacterial infections.

How is REG3A being explored in cancer research? 10/14/2020

REG3A's involvement in cell growth and differentiation has led to investigations into its role in cancer, with studies exploring its potential as a therapeutic target or diagnostic marker.

Customer Reviews (3)

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Reviews
02/01/2022

    REG3A protein is of exceptional quality and is highly recommended for meeting experimental needs.

    12/16/2020

      Its purity and integrity ensure reliable and reproducible results in a wide range of applications.

      11/01/2017

        the manufacturer of REG3A protein provides excellent technical support, ensuring prompt assistance and guidance.

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