Recombinant Human REG3A cell lysate
Cat.No. : | REG3A-2496HCL |
Product Overview : | Human REG3A / HIP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | REG3A regenerating islet-derived 3 alpha [ Homo sapiens ] |
Official Symbol : | REG3A |
Synonyms : | REG3A; regenerating islet-derived 3 alpha; pancreatitis associated protein , PAP; regenerating islet-derived protein 3-alpha; HIP; PAP1; PBCGF; REG III; REG3; REG-3-alpha; reg III-alpha; PAP homologous protein; human proislet peptide; pancreatitis-associated protein 1; proliferation-inducing protein 34; proliferation-inducing protein 42; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; regenerating islet-derived protein III-alpha; PAP; PAP-H; REG-III; FLJ18565; |
Gene ID : | 5068 |
mRNA Refseq : | NM_002580 |
Protein Refseq : | NP_002571 |
MIM : | 167805 |
UniProt ID : | Q06141 |
Chromosome Location : | 2p12 |
Function : | binding; sugar binding; |
Products Types
◆ Recombinant Protein | ||
REG3A-2650H | Recombinant Human REG3A Protein, His-tagged | +Inquiry |
REG3A-4647R | Recombinant Rat REG3A Protein, His (Fc)-Avi-tagged | +Inquiry |
REG3A-431H | Recombinant Human REG3A Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
REG3A-86H | Active Recombinant Human REG3A Protein | +Inquiry |
REG3A-2337H | Recombinant Human REG3A protein(Met1-Asp175), His-tagged | +Inquiry |
◆ Lysates | ||
REG3A-2691MCL | Recombinant Mouse REG3A cell lysate | +Inquiry |
REG3A-1326RCL | Recombinant Rat REG3A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionREG3A has been associated with inflammatory bowel diseases (IBD), such as Crohn's disease and ulcerative colitis, where its dysregulation can contribute to inflammation and tissue damage.
REG3A plays a role in maintaining the balance of the gut microbiota by influencing the composition of the microbial community.
Yes, altered levels of REG3A have been observed in several diseases, making it a potential biomarker for gastrointestinal disorders and certain cancers.
REG3A has immunomodulatory effects, participating in the regulation of the immune response, particularly in the defense against bacterial infections.
REG3A's involvement in cell growth and differentiation has led to investigations into its role in cancer, with studies exploring its potential as a therapeutic target or diagnostic marker.
Customer Reviews (3)
Write a reviewREG3A protein is of exceptional quality and is highly recommended for meeting experimental needs.
Its purity and integrity ensure reliable and reproducible results in a wide range of applications.
the manufacturer of REG3A protein provides excellent technical support, ensuring prompt assistance and guidance.
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