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Recombinant Mouse IL13RA1 cell lysate

Cat.No. : IL13RA1-2627MCL
Product Overview : Mouse IL13Ra1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il13ra1 interleukin 13 receptor, alpha 1 [ Mus musculus ]
Official Symbol : IL13RA1
Synonyms : IL13RA1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; IL-13RA1; IL-13R-alpha-1; IL-13R subunit alpha-1; novel cytokine receptor 4; IL-13 receptor subunit alpha-1; interleukin-13-binding protein; NR4; Il13ra; CD213a1; AI882074; IL-13r[a];
Gene ID : 16164
mRNA Refseq : NM_133990
Protein Refseq : NP_598751
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-4 signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : cytokine receptor activity; interleukin-13 receptor activity; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques determine IL13RA1 expression levels in different cell types? 12/05/2022

Techniques quantify IL13RA1 expression across diverse cell types.

What experimental strategies discern IL13RA1 isoforms and their functions? 10/04/2021

Strategies distinguish IL13RA1 isoforms and their roles.

Could you elaborate on the use of RNA-seq in profiling IL13RA1-regulated gene expression? 04/01/2021

RNA-seq profiles IL13RA1's influence on gene expression.

What are the advantages of using CRISPR/Cas9 to knockout IL13RA1 expression? 05/10/2020

CRISPR/Cas9 knockout clarifies IL13RA1's functional impact.

How do siRNA knockdown experiments validate IL13RA1's involvement in specific pathways? 03/08/2020

siRNA knockdown validates IL13RA1's pathway involvement.

Can you explain the role of co-immunoprecipitation in identifying IL13RA1 interaction partners? 01/17/2020

Co-immunoprecipitation identifies IL13RA1 interactors.

How does immunofluorescence microscopy visualize IL13RA1 localization within cells? 05/22/2019

Immunofluorescence microscopy visualizes intracellular IL13RA1 localization.

How does surface plasmon resonance (SPR) elucidate IL13RA1 binding kinetics? 10/08/2018

SPR elucidates IL13RA1 binding kinetics with precision.

Can you describe how surface biotinylation studies reveal IL13RA1 membrane localization? 09/25/2018

Surface biotinylation shows IL13RA1 membrane distribution.

How do fluorescence-based assays assess IL13RA1 internalization upon ligand binding? 06/19/2018

Fluorescence assays monitor IL13RA1 internalization upon ligand binding.

Customer Reviews (3)

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Reviews
01/10/2022

    High solubility.

    01/17/2019

      Potent in anti-allergic assays.

      04/26/2018

        Suitable for ELISA.

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