| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Amino Acid sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity : |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation : |
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10 mM sodium Citrate PH 3.0. |
| Solubility : |
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18 MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Specific Activity : |
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3µg/ml in the presence of 15 ng/ml of Human IGF-II. |
| Stability : |
Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
