Recombinant Human IL1RAP cell lysate
Cat.No. : | IL1RAP-2730HCL |
Product Overview : | Human IL1R3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | IL1RAP interleukin 1 receptor accessory protein [ Homo sapiens ] |
Official Symbol : | IL1RAP |
Synonyms : | IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; C3orf13; IL 1RAcP; IL1R3; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; IL-1RAcP; FLJ37788; |
Gene ID : | 3556 |
mRNA Refseq : | NM_001167928 |
Protein Refseq : | NP_001161400 |
MIM : | 602626 |
UniProt ID : | Q9NPH3 |
Chromosome Location : | 3q28 |
Pathway : | Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Immune System, organism-specific biosystem; |
Function : | interleukin-1 receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionSingle-cell RNA sequencing and proteomics unveil IL1RAP's interactions in networks.
Genome mapping and assays unveil IL1RAP's epigenetic control.
Innovative methods clarify IL1RAP's distribution diversity, revealing its functional adaptability.
Advanced imaging and genetic models illuminate IL1RAP's immune role.
Simulations and experiments reveal IL1RAP's interaction details.
Engineered cell models shed light on IL1RAP's role in inflammation.
Structural techniques provide insights into IL1RAP's ligand interactions.
Strategies uncover post-translational effects on IL1RAP's signaling.
Organ-on-chip tech explores IL1RAP's impact on pathways.
Novel techniques decode IL1RAP isoforms, exposing their influence on cell responses.
Customer Reviews (3)
Write a reviewHigh solubility.
Good for intracellular staining.
Effective in apoptosis assays.
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