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Recombinant Mouse LCN2 cell lysate

Cat.No. : LCN2-2781MCL
Product Overview : Mouse LCN2 / NGAL derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Lcn2 lipocalin 2 [ Mus musculus ]
Official Symbol : LCN2
Synonyms : LCN2; lipocalin 2; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; SV-40-induced 24P3 protein; secreted inducible protein 24; 24p3; Sip24; AW212229;
Gene ID : 16819
mRNA Refseq : NM_008491
Protein Refseq : NP_032517
Function : binding; iron ion binding; protease binding; protein binding; protein homodimerization activity; transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What diseases are associated with mutations or abnormal expression of the LCN2 protein? 06/19/2022

Mutations or abnormal expression of the LCN2 protein may be associated with a variety of diseases. For example, in hereditary kidney disease, mutations in the uNGAL gene can lead to kidney damage. In addition, in inflammatory diseases, tumors, and other diseases, the expression level of LCN2 protein may also change.

What diseases are LCN2 protein expression levels associated with? 11/15/2020

Studies have shown that the expression level of LCN2 protein is associated with a variety of diseases. For example, in kidney disease, expression levels of the LCN2 protein can be elevated and can be used as one of the biomarkers to assess the extent of kidney damage. In addition, in inflammatory diseases, tumors, and other diseases, the expression level of LCN2 protein can also change.

What is the interaction between the LCN2 protein and other proteins or molecules? 07/22/2020

The interaction between the LCN2 protein and other proteins or molecules is complex. For example, it can participate in lipid metabolism processes by interacting with lipid molecules. In addition, it can interact with other signal transduction molecules to participate in the signal transduction process.

How can the expression level of LCN2 protein be used for disease diagnosis? 04/03/2020

Kidney function can be assessed by measuring the level of uNGAL in urine, and to help diagnose kidney disease, etc. In addition, abnormal levels of LCN2 protein expression can also be used as an indicator to assess the risk and prognosis of other diseases.

How is the activity of LCN2 protein regulated? 02/16/2020

The activity of the LCN2 protein is regulated by a variety of factors. First, its expression level is regulated by gene expression. Secondly, its activity is also regulated by a variety of small molecule compounds, hormones and other substances. In addition, the LCN2 protein can also regulate its activity by interacting with other proteins.

How does the LCN2 protein relate to other biomarkers? 06/06/2019

LCN2 protein has some association with other biomarkers. For example, in kidney disease, expression levels of uNGAL can be used alongside traditional biomarkers such as creatinine to assess kidney function and degree of impairment. In addition, in diseases such as tumors, expression levels of the LCN2 protein may also change and be associated with other biomarkers.

Customer Reviews (3)

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Reviews
05/02/2022

    They have a wide range of products and can always find LCN2 we need.

    03/11/2021

      The reproducibility of LCN2 is almost 100 percent, and the results are very consistent in every experiment or application, which is an important reflection of its excellent quality.

      12/15/2019

        Catalytic activity of LCN2 is really amazing, and every time it is used, it gives an unexpected catalytic effect and its performance is impressive.

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