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Recombinant Mouse SFRP2 cell lysate

Cat.No. : SFRP2-2851MCL
Product Overview : Mouse sFRP2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Sfrp2 secreted frizzled-related protein 2 [ Mus musculus ]
Official Symbol : SFRP2
Synonyms : SFRP2; secreted frizzled-related protein 2; SARP-1; sFRP-2; stromal cell derived factor 5; secreted apoptosis-related protein 1; secreted frizzled-related sequence protein 2; secreted frizzled-related sequence protein 5; Sdf5; AI851596;
Gene ID : 20319
mRNA Refseq : NM_009144
Protein Refseq : NP_033170
Pathway : Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem;
Function : PDZ domain binding; Wnt-activated receptor activity; Wnt-protein binding; endopeptidase activator activity; metalloenzyme activator activity; protein binding; receptor agonist activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does SFRP2 expression influence stem cell differentiation and regeneration in various tissues? 01/11/2022

SFRP2 expression plays a significant role in stem cell differentiation, contributing to tissue regeneration and repair processes.

What role does SFRP2 play in the development and progression of cardiovascular diseases, especially atherosclerosis? 08/22/2021

In cardiovascular diseases, SFRP2 plays a role in processes like atherosclerosis, impacting vascular health and disease progression.

What is the impact of SFRP2 on angiogenesis in the context of tumor growth and metastasis? 02/20/2021

SFRP2 influences angiogenesis in tumors, affecting tumor growth and the potential for metastasis.

How does SFRP2 interact with Wnt signaling pathways in cancer development, particularly in colorectal and breast cancers? 11/06/2020

SFRP2 modulates Wnt signaling in cancers, with specific roles in colorectal and breast cancer development, impacting cell proliferation and migration.

How does SFRP2 modulate bone formation and resorption in osteoporosis and other bone diseases? 05/24/2020

SFRP2 is involved in bone metabolism, affecting bone formation and resorption in diseases like osteoporosis.

What are the mechanisms by which SFRP2 contributes to fibrosis in organ systems like the heart and liver? 03/02/2020

SFRP2 contributes to fibrosis in organs like the heart and liver, influencing tissue remodeling and function.

How does the alteration of SFRP2 expression affect adipogenesis and metabolic processes in obesity and diabetes? 11/17/2018

Alterations in SFRP2 expression affect adipogenesis and metabolic functions, linking it to obesity and diabetes pathologies.

Customer Reviews (3)

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Reviews
11/13/2021

    Fast and affordable protein sequencing; great for identifying unknown proteins.

    04/28/2019

      Robust protein-protein interaction assays; pivotal for our experiments.

      02/20/2018

        High-quality protein crystallization; crucial for structural biology studies.

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