Recombinant Mouse SFRP2 cell lysate
Cat.No. : | SFRP2-2851MCL |
Product Overview : | Mouse sFRP2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Sfrp2 secreted frizzled-related protein 2 [ Mus musculus ] |
Official Symbol : | SFRP2 |
Synonyms : | SFRP2; secreted frizzled-related protein 2; SARP-1; sFRP-2; stromal cell derived factor 5; secreted apoptosis-related protein 1; secreted frizzled-related sequence protein 2; secreted frizzled-related sequence protein 5; Sdf5; AI851596; |
Gene ID : | 20319 |
mRNA Refseq : | NM_009144 |
Protein Refseq : | NP_033170 |
Pathway : | Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem; |
Function : | PDZ domain binding; Wnt-activated receptor activity; Wnt-protein binding; endopeptidase activator activity; metalloenzyme activator activity; protein binding; receptor agonist activity; |
Products Types
◆ Recombinant Protein | ||
SFRP2-4481H | Recombinant Human SFRP2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Sfrp2-008M | Recombinant Mouse Sfrp2 Protein, His-tagged | +Inquiry |
Sfrp2-8085M | Recombinant Mouse Sfrp2 Protein, His (Fc)-Avi-tagged | +Inquiry |
SFRP2-762C | Recombinant Cynomolgus SFRP2 protein, His-tagged | +Inquiry |
SFRP2-4716Z | Recombinant Zebrafish SFRP2 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionSFRP2 expression plays a significant role in stem cell differentiation, contributing to tissue regeneration and repair processes.
In cardiovascular diseases, SFRP2 plays a role in processes like atherosclerosis, impacting vascular health and disease progression.
SFRP2 influences angiogenesis in tumors, affecting tumor growth and the potential for metastasis.
SFRP2 modulates Wnt signaling in cancers, with specific roles in colorectal and breast cancer development, impacting cell proliferation and migration.
SFRP2 is involved in bone metabolism, affecting bone formation and resorption in diseases like osteoporosis.
SFRP2 contributes to fibrosis in organs like the heart and liver, influencing tissue remodeling and function.
Alterations in SFRP2 expression affect adipogenesis and metabolic functions, linking it to obesity and diabetes pathologies.
Customer Reviews (3)
Write a reviewFast and affordable protein sequencing; great for identifying unknown proteins.
Robust protein-protein interaction assays; pivotal for our experiments.
High-quality protein crystallization; crucial for structural biology studies.
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