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Recombinant Mouse IGFBP4 cell lysate

Cat.No. : IGFBP4-2925MCL
Product Overview : Mouse IGFBP4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Igfbp4 insulin-like growth factor binding protein 4 [ Mus musculus ]
Official Symbol : IGFBP4
Synonyms : IGFBP4; insulin-like growth factor binding protein 4; insulin-like growth factor-binding protein 4; IBP-4; Band 3A; IGF-binding protein 4; differentially expressed in B16F10 2; Deb2; IGFBP-4; AI875747;
Gene ID : 16010
mRNA Refseq : NM_010517
Protein Refseq : NP_034647
Pathway : Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem;
Function : growth factor binding; insulin-like growth factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does IGFBP4 play in bone growth and development, particularly in regulating osteoblast and osteoclast activity? 09/09/2022

IGFBP4 plays a key role in bone development, regulating bone-forming and bone-resorbing cells.

What is the impact of IGFBP4 on cardiovascular health, particularly in atherosclerosis and heart disease? 04/30/2022

IGFBP4 impacts cardiovascular health, influencing atherosclerosis and heart disease processes.

What are the effects of IGFBP4 dysregulation on aging and age-related diseases? 10/08/2019

Dysregulation of IGFBP4 is linked to aging and the progression of age-related diseases.

How does IGFBP4 contribute to metabolic regulation and insulin sensitivity? 11/25/2018

IGFBP4 is involved in metabolic regulation, affecting insulin sensitivity and glucose metabolism.

How does IGFBP4 modulate the activity of insulin-like growth factors (IGFs) in various tissues? 10/23/2018

IGFBP4 modulates IGF activity, affecting growth and metabolic functions in tissues.

How do alterations in IGFBP4 expression or function affect wound healing and tissue repair? 07/09/2018

Changes in IGFBP4 expression influence wound healing and tissue repair mechanisms.

How does IGFBP4 influence cancer progression, especially in hormone-dependent cancers? 05/26/2018

IGFBP4 influences cancer progression, particularly in cancers sensitive to hormonal changes.

Customer Reviews (3)

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Reviews
02/25/2019

    Accurate protein structural analysis, crucial for our investigations.

    10/23/2018

      Exceptional protein expression optimization support, greatly appreciated.

      06/06/2018

        Reliable mass spectrometry analysis, pivotal for proteomics studies.

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