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Recombinant Human DPP4 cell lysate

Cat.No. : DPP4-2979HCL
Product Overview : Human DPPIV / DPP4 / CD26 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : DPP4 dipeptidyl-peptidase 4 [ Homo sapiens ]
Official Symbol : DPP4
Synonyms : DPP4; dipeptidyl-peptidase 4; ADCP2, adenosine deaminase complexing protein 2 , CD26, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl peptidase 4; DPPIV; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); CD26; ADABP; ADCP2; TP103;
Gene ID : 1803
mRNA Refseq : NM_001935
Protein Refseq : NP_001926
MIM : 102720
UniProt ID : P27487
Chromosome Location : 2q23-qter
Pathway : Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Synthesis, Secretion, and Inactivation of Glucagon-like Peptide-1 (GLP-1), organism-specific biosystem;
Function : aminopeptidase activity; collagen binding; dipeptidyl-peptidase activity; peptidase activity; peptide binding; protease binding; protein binding; protein homodimerization activity; receptor activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does DPP4 relate to diabetes? 04/17/2023

DPP4 plays a crucial role in glucose homeostasis by cleaving incretin hormones. In diabetes, inhibiting DPP4 can enhance the action of these hormones, promoting insulin release and lowering blood sugar levels.

Are there any side effects associated with DPP4 inhibitors? 01/11/2022

Common side effects of DPP4 inhibitors include respiratory and urinary tract infections, headache, and nasopharyngitis. However, they are generally well-tolerated.

What clinical applications does DPP4 have in managing type 2 diabetes? 06/12/2020

Inhibitors of DPP4 are used in the treatment of type 2 diabetes to improve glycemic control. They work by prolonging the activity of incretin hormones, such as GLP-1, which stimulate insulin secretion.

Can DPP4 inhibitors be used as a monotherapy for diabetes? 03/10/2020

DPP4 inhibitors are often used as monotherapy or in combination with other antidiabetic medications to achieve optimal glycemic control.

How do DPP4 inhibitors differ from other diabetes medications? 06/11/2016

DPP4 inhibitors work by enhancing the effects of incretin hormones, while other diabetes medications may act on insulin sensitivity, insulin release, or glucose absorption.

Customer Reviews (3)

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Reviews
09/13/2019

    Its purity and efficacy make it an ideal choice for precise and accurate analysis, allowing me to generate reliable results.

    01/27/2019

      By incorporating the DPP4 Protein into their studies, scientists can confidently conduct their research, ushering in groundbreaking discoveries and novel insights within their respective domains.

      12/14/2016

        Its exceptional purity and functionality make it an ideal choice for accurate and precise analysis, ensuring reliable and reproducible results.

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