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Recombinant Human CSF2 cell lysate

Cat.No. : CSF2-3008HCL
Product Overview : Human GM-CSF / CSF2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
Official Symbol : CSF2
Synonyms : CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; GM CSF; GMCSF; granulocyte macrophage colony stimulating factor; molgramostin; sargramostim; CSF; colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897;
Gene ID : 1437
mRNA Refseq : NM_000758
Protein Refseq : NP_000749
MIM : 138960
UniProt ID : P04141
Chromosome Location : 5q23-q31
Pathway : Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How conserved is the sequence of CSF2 among different species? 05/15/2021

The sequence of CSF2 is moderately conserved across different species.

What is the typical length of CSF2? 01/30/2021

The length of CSF2 protein varies but is typically around 144 amino acids.

What is the main biological function of CSF2? 05/01/2019

The primary function of CSF2 is to regulate the production and differentiation of white blood cells.

In what tissues or cells is CSF2 commonly expressed? 02/03/2019

CSF2 is often expressed in immune cells, specifically in macrophages, T cells, and endothelial cells.

What does CSF2 stand for? 07/10/2018

CSF2 stands for Colony Stimulating Factor 2.

Is CSF2 associated with any specific diseases or conditions? 03/17/2018

CSF2 dysregulation has been linked to autoimmune diseases and inflammatory disorders.

Which cellular functions are associated with CSF2? 02/20/2017

CSF2 is involved in the stimulation of stem cells to produce granulocytes and monocytes.

Customer Reviews (3)

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Reviews
01/15/2022

    The product's adherence to regulatory standards aligned with our lab's commitment to responsible and transparent research.

    07/27/2021

      The manufacturer's commitment to meeting regulatory requirements demonstrated its dedication to ethical research practices.

      05/19/2019

        Compliance with industry standards minimized potential roadblocks in securing approvals for our experiments.

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