Recombinant Human BMP5 cell lysate
Cat.No. : | BMP5-3074HCL |
Product Overview : | Human BMP-5 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | BMP5 bone morphogenetic protein 5 [ Homo sapiens ] |
Official Symbol : | BMP5 |
Synonyms : | BMP5; bone morphogenetic protein 5; BMP-5; MGC34244; |
Gene ID : | 653 |
mRNA Refseq : | NM_021073 |
Protein Refseq : | NP_066551 |
MIM : | 112265 |
UniProt ID : | P22003 |
Chromosome Location : | 6p12.1 |
Pathway : | Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem; |
Function : | BMP receptor binding; cytokine activity; growth factor activity; |
Products Types
◆ Recombinant Protein | ||
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BMP5-4365H | Recombinant Human BMP5 Protein, His (Fc)-Avi-tagged | +Inquiry |
Bmp5-260M | Recombinant Mouse Bmp5 Protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionBMP5, a member of the bone morphogenetic protein family, functions by binding to cell surface receptors and activating the Smad signaling pathway. This leads to the phosphorylation and nuclear localization of transcription factors, thereby regulating gene expression. BMP5 plays a crucial role in cellular proliferation, differentiation, and fate determination. Its signaling mechanism involves various molecular regulations, including the involvement of co-receptors, signal transduction mediators, and downstream effectors.
BMP5 regulates the expression of various downstream target genes involved in multiple cellular processes. These include genes encoding transcription factors, extracellular matrix components, growth factors, and signaling molecules. Examples of BMP5 target genes include Runx2, Osterix, Sox9, Noggin, and Gremlin. Through the activation of these target genes, BMP5 influences cell fate determination, osteoblast differentiation, chondrogenesis, and tissue homeostasis.
BMP5 plays a critical role in skeletal development and bone formation. It promotes the commitment of mesenchymal stem cells to the osteoblast lineage and stimulates their differentiation into mature osteoblasts. BMP5 also regulates the proliferation and survival of osteoblasts, as well as the synthesis and mineralization of the extracellular matrix. Additionally, BMP5 interacts with other BMP family members and signaling molecules to coordinate the complex processes of skeletal patterning, limb development, and bone remodeling.
Dysregulation of BMP5 expression or signaling has been implicated in various skeletal disorders, including osteoporosis, osteoarthritis, and skeletal dysplasias. BMP5 mutations or alterations in its expression levels can disrupt normal bone development and contribute to skeletal abnormalities. Additionally, BMP5 has been found to play a role in cancer progression. Depending on the context, BMP5 can act as either a tumor suppressor or a promoter by influencing cell proliferation, apoptosis, angiogenesis, and metastasis.
Despite significant progress, several challenges remain in BMP5 research. Understanding the precise mechanisms underlying BMP5 signaling and its cross-talk with other signaling pathways is crucial. Additionally, investigating the context-dependent functions of BMP5 in different tissues and disease conditions is important for developing targeted therapies. Future research should focus on elucidating the regulatory networks controlling BMP5 expression and identifying novel therapeutic targets within the BMP5 signaling pathway. Moreover, studying the potential of BMP5 as a diagnostic or prognostic marker in various diseases would further expand its clinical relevance.
The expression of BMP5 is tightly regulated through a combination of transcriptional, post-transcriptional, and post-translational mechanisms. Transcription factors, such as Smads, homeobox proteins, and other tissue-specific regulators, bind to the promoter region of the BMP5 gene, modulating its expression. Additionally, microRNAs and RNA-binding proteins can influence the stability and translation of BMP5 mRNA. Furthermore, epigenetic modifications, including DNA methylation and histone acetylation, play a role in regulating BMP5 expression during development and in response to environmental cues.
Targeting BMP5 signaling holds therapeutic potential for various diseases. For example, in bone-related disorders, modulating BMP5 activity could be explored to promote bone regeneration or inhibit excessive bone loss. In cancer, therapeutic strategies targeting BMP5 signaling could be designed to regulate tumor growth, metastasis, or sensitize cancer cells to existing therapies. However, further research is needed to fully understand the complex role of BMP5 in disease pathogenesis and to develop targeted therapies that specifically modulate BMP5 activity.
Customer Reviews (3)
Write a reviewRevolutionizing the art of experimentation, this reagent blazes a trail of innovation.
By using this reagent, I am able to delve deeper into my research topics, deriving more persuasive conclusions.
The reagent showcases remarkable versatility and unwavering stability when it comes to the manipulation of intricate samples.
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