Recombinant Human CSF2, His-tagged

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Recombinant Human CSF2, His-tagged

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Cat. No. : CSF2-87H
Product Overview : Recombinant Human GM-CSF produced ininsect cellsis a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.
Description : GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Source : Insect Cells.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (c) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized with no additives.
Solubility : It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity : The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x10⁷ IU/mg.
Protein Content : Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequence (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GMCSF as a Reference Standard.
Stability : Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
PDB rendering based on 2gmf :

Gene Name : CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
Synonyms : CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; MGC131935; MGC138897; colony stimulating factor 2; molgramostin; sargramostim; granulocyte-macrophage colony stimulating factor; GM-CSF; Molgramostin; Sargramostim; Colony-stimulating factor
Gene ID : 1437
mRNA Refseq : NM_000758
Protein Refseq : NP_000749
MIM : 138960
UniProt ID : P04141
Chromosome Location : 5q31.1
Pathway : Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; T cell receptor signaling pathway
Function : cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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