Recombinant Human CLOCK, GST-tagged

• Cat.No.: CLOCK-75H
• Product Overview: Recombinant Human CLOCK(1 a.a. - 846 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 119.5 kDa
• AA Sequence: MLFTVSCSKMSSIVDRDDSSIFDGL VEEDDKDKAKRVSRNKSEKKRRDQF NVLIKELGSMLPGNARKMDKSTVLQ KSIDFLRKHKEITAQSDASEIRQDW KPTFISNEEFTQLMLEALDGFFLAI MTDGSIIYVSESVTSLLEHLPSDLV DQSIFNFIPEGEHSEVYKILSTHLL ESDSLTPEYLKSKNQLEFCCHMLRG TIDPKEPSTYEYVKFIGNFKSLNSV SSSAHNGFEGTIQRTHRPSYEDRVC FVATVRLATPQFIKEMCTVEEPNEE FTSRHSLEWKFLFLDHRAPPIIGYL PFEVLGTSGYDYYHVDDLENLAKCH EHLMQYGKGKSCYYRFLTKGQQWIW LQTHYYITYHQWNSRPEFIVCTHTV VSYAEVRAERRRELGIEESLPETAA DKSQDSGSDNRINTVSLKEALERFD HSPTPSASSRSSRKSSHTAVSDPSS TPTKIPTDTSTPPRQHLPAHEKMVQ RRSSFSSQSINSQSVGSSLTQPVMS QATNLPIPQGMSQFQFSAQLGAMQH LKDQLEQRTRMIEANIHRQQEELRK IQEQLQMVHGQGLQMFLQQSNPGLN FGSVQLSSGNSSNIQQLAPINMQGQ VVPTNQIQSGMNTGHIGTTQHMIQQ QTLQSTSTQSQQNVLSGHSQQTSLP SQTQSTLTAPLYNTMVISQPAAGSM VQIPSSMPQNSTQSAAVTTFTQDRQ IRFSQGQQLVTKLVTAPVACGAVMV PSTMLMGQVVTAYPTFATQQQQSQT LSVTQQQQQQSSQEQQLTSVQQPSQ AQLTQPPQQFLQTSRLLHGNPSTQL ILSAAFPLQQSTFPQSHHQQHQSQQ QQQLSRHRTDSLPDPSKVQPQ
• Applications: ELISA; WB-Re; AP; Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CLOCK clock circadian regulator [ Homo sapiens (human) ]
• Official Symbol: CLOCK
• Synonyms: CLOCK; clock homolog (mouse); clock (mouse) homolog; circadian locomoter output cycles protein kaput; bHLHe8; KAT13D; KIAA0334; class E basic helix-loop-helix protein 8; circadian locomoter output cycles kaput protein
• Gene ID: 9575
• mRNA Refseq: NM_004898
• Protein Refseq: NP_004889
• MIM: 601851
• UniProt ID: O15516
• Chromosome Location: 4q12
• Pathway: BMAL1:CLOCK,NPAS2 activates circadian gene expression; Chromatin modifying enzymes; Circadian Clock
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; chromatin DNA binding