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Recombinant Human ZCCHC7 293 Cell Lysate

Cat.No.: ZCCHC7-200HCL
Description: Antigen standard for zinc finger, CCHC domain containing 7 (ZCCHC7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ZCCHC7 zinc finger, CCHC domain containing 7 [ Homo sapiens ]
Official Symbol: ZCCHC7
Synonyms: ZCCHC7; zinc finger, CCHC domain containing 7; zinc finger CCHC domain-containing protein 7; AIR1; FLJ22611; HSPC086; RP11-397D12.1;
Gene ID: 84186
mRNA Refseq: NM_032226
Protein Refseq: NP_115602
UniProt ID: Q8N3Z6
Chromosome Location: 9p13.1
Pathway: RNA degradation, organism-specific biosystem; RNA degradation, conserved biosystem; TRAMP complex, organism-specific biosystem; TRAMP complex, conserved biosystem;

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