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Recombinant Human XRCC4 293 Cell Lysate

Cat.No. : XRCC4-255HCL
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  • Gene Information
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Description : Antigen standard for X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : XRCC4 X-ray repair complementing defective repair in Chinese hamster cells 4 [ Homo sapiens ]
Official Symbol : XRCC4
Synonyms : XRCC4; X-ray repair complementing defective repair in Chinese hamster cells 4; DNA repair protein XRCC4; X ray repair; complementing defective; repair in Chinese hamster; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster;
Gene ID : 7518
mRNA Refseq : NM_003401
Protein Refseq : NP_003392
MIM : 194363
UniProt ID : Q13426
Chromosome Location : 5q14.2
Pathway : 2-LTR circle formation, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the challenges in targeting XRCC4 for cancer therapy? 11/12/2022

Resistance mechanisms and potential side effects of XRCC4-targeted therapies pose challenges in cancer treatment.

How can XRCC4 protein be targeted in cancer therapy? 09/22/2021

Understanding XRCC4 function may lead to the development of targeted therapies to enhance cancer treatment efficacy.

Can XRCC4 protein be a target for drug development? 06/20/2021

XRCC4 is a potential target for the development of novel cancer therapies aimed at disrupting DNA repair mechanisms.

Are there diagnostic applications of XRCC4 protein? 07/07/2017

XRCC4 levels or mutations may have potential as biomarkers for certain cancers and their response to treatment.

Are there genetic variations in XRCC4 that impact cancer risk? 03/26/2016

Certain genetic variations in XRCC4 have been associated with an increased risk of developing certain types of cancer.

Customer Reviews (3)

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Reviews
11/20/2022

    Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

    08/27/2022

      Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

      08/21/2019

        With the manufacturer's excellent technical support and its wide range of applications, the XRCC4 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

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