"XRCC2" Related Products

Recombinant Human XRCC2 293 Cell Lysate

Cat.No.: XRCC2-257HCL
Description: Antigen standard for X-ray repair complementing defective repair in Chinese hamster cells 2 (XRCC2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: XRCC2 X-ray repair complementing defective repair in Chinese hamster cells 2 [ Homo sapiens ]
Official Symbol: XRCC2
Synonyms: XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; DNA repair protein XRCC2; RAD51 like; X-ray repair cross-complementing protein 2; X-ray repair, complementing defective, repair in Chinese hamster; DKFZp781P0919;
Gene ID: 7516
mRNA Refseq: NM_005431
Protein Refseq: NP_005422
MIM: 600375
UniProt ID: O43543
Chromosome Location: 7q36
Pathway: Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem;

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