"VWA5A" Related Products

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Recombinant Human VWA5A 293 Cell Lysate

Cat.No.: VWA5A-373HCL
Description: Antigen standard for von Willebrand factor A domain containing 5A (VWA5A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: VWA5A von Willebrand factor A domain containing 5A [ Homo sapiens ]
Official Symbol: VWA5A
Synonyms: VWA5A; von Willebrand factor A domain containing 5A; LOH11CR2A, loss of heterozygosity, 11, chromosomal region 2, gene A; von Willebrand factor A domain-containing protein 5A; BCSC 1; ortholog of mouse AW551984; breast cancer suppressor candidate 1; loss of heterozygosity, 11, chromosomal region 2, gene A; loss of heterozygosity 11 chromosomal region 2 gene A protein; BCSC1; BCSC-1; LOH11CR2A;
Gene ID: 4013
mRNA Refseq: NM_198315
Protein Refseq: ?NP_938057
MIM: 602929
UniProt ID: O00534
Chromosome Location: 11q24.1

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