Cat.No.:	USP20-467HCL
Description:	Antigen standard for ubiquitin specific peptidase 20 (USP20), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source:	HEK 293 cells
Species:	Human
Components:	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size:	0.1 mg
Storage Instruction:	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications:	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name:	USP20 ubiquitin specific peptidase 20 [ Homo sapiens ]
Official Symbol:	USP20
Synonyms:	USP20; ubiquitin specific peptidase 20; ubiquitin specific protease 20; ubiquitin carboxyl-terminal hydrolase 20; KIAA1003; hVDU2; ubiquitin thioesterase 20; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; VHL-interacting deubiquitinating enzyme 2; ubiquitin-specific-processing protease 20; pVHL-interacting deubiquitinating enzyme 2; VDU2; LSFR3A;
Gene ID:	10868
mRNA Refseq:	NM_001008563
Protein Refseq:	NP_001008563
UniProt ID:	Q9Y2K6
Chromosome Location:	9q34.2

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