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Recombinant Human USP13 293 Cell Lysate

Cat.No. : USP13-472HCL
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Description : Antigen standard for ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : USP13 ubiquitin specific peptidase 13 (isopeptidase T-3) [ Homo sapiens ]
Official Symbol : USP13
Synonyms : USP13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T 3); ubiquitin carboxyl-terminal hydrolase 13; IsoT 3; isopeptidase T-3; ubiquitin thioesterase 13; deubiquitinating enzyme 13; ubiquitin thiolesterase 13; ubiquitin-specific-processing protease 13; ubiquitin specific protease 13 (isopeptidase T-3); ISOT3; IsoT-3;
Gene ID : 8975
mRNA Refseq : NM_003940
Protein Refseq : NP_003931
MIM : 603591
UniProt ID : Q92995
Chromosome Location : 3q26.2-q26.3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can USP13 be a potential therapeutic target for neurodegenerative diseases? 02/09/2022

There is emerging research suggesting that USP13 may play a role in neurodegenerative diseases, and targeting it could be a potential avenue for therapeutic intervention.

How does USP13 interact with other proteins in cellular pathways? 07/26/2021

USP13 interacts with various proteins, forming complexes that regulate different cellular pathways. Understanding these interactions is crucial for deciphering its role in health and disease.

Are there any known genetic variations in the USP13 gene associated with disease susceptibility? 02/17/2021

Genetic variations in the USP13 gene have been studied, and certain polymorphisms may be associated with an increased risk of specific diseases. Further research is ongoing in this area.

In what other medical conditions is USP13 protein being investigated? 03/22/2019

Researchers are exploring the role of USP13 in neurodegenerative diseases, inflammatory disorders, and other conditions where ubiquitin-mediated processes play a crucial role.

How does USP13 influence cellular response to stress and DNA damage? 04/03/2016

USP13 has been found to regulate the stability of proteins involved in the DNA damage response, affecting how cells respond to stress and maintain genomic integrity.

Customer Reviews (3)

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Reviews
01/02/2023

    Its purity, integrity, and consistency ensure reliable and reproducible results, which are essential for meaningful scientific discoveries.

    06/29/2016

      Their in-depth understanding of the protein's characteristics, applications, and potential limitations allows for valuable insights and recommendations, ensuring the success of my experiments.

      04/07/2016

        Whether it's troubleshooting, protocol optimization, or general inquiries, their knowledgeable and responsive team is readily available to assist and solve any problems that may arise during the experimental process.

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