Recombinant Human UCK2 293 Cell Lysate

• Cat.No.: UCK2-531HCL

Specification

• Description: Antigen standard for uridine-cytidine kinase 2 (UCK2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: UCK2 uridine-cytidine kinase 2 [ Homo sapiens ]
• Official Symbol: UCK2
• Synonyms: UCK2; uridine-cytidine kinase 2; UMPK, uridine monophosphate kinase; uridine kinase; uridine monophosphokinase 2; cytidine monophosphokinase 2; uridine monophosphate kinase; testis-specific protein TSA903; UK; UMPK; TSA903; DKFZp686M04245
• Gene ID: 7371
• mRNA Refseq: NM_012474
• Protein Refseq: NP_036606
• MIM: 609329
• UniProt ID: Q9BZX2
• Chromosome Location: 1p32
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem

Reviews

08/24/2022

I highly endorse the utilization of the UCK2 Protein for researchers seeking a superior protein solution that not only meets their experimental needs but also benefits from the exceptional technical support provided by the manufacturer.

10/09/2021

Their expertise has proven instrumental in overcoming obstacles and has greatly contributed to the success of my research endeavors.

05/07/2020

Their team of experts has been extremely helpful in addressing any concerns or queries I encountered during my experiments.

Q&As

Can UCK2 protein levels in patient samples be measured for clinical assessment?

Yes, UCK2 protein levels can be quantified in patient samples using techniques such as immunohistochemistry and ELISA for clinical assessment.

Are there any genetic mutations associated with UCK2 protein that impact clinical outcomes?

Certain genetic mutations in the UCK2 gene have been linked to altered UCK2 protein function, which may impact clinical outcomes in certain diseases.

Does UCK2 protein have any role in neurological disorders?

Some studies have suggested a potential link between UCK2 protein and neurological disorders, although further research is needed to fully understand this association.

How is UCK2 protein involved in liver diseases?

UCK2 protein has been implicated in liver diseases, particularly in the context of nucleoside analog metabolism and liver cell function.

What role does UCK2 protein play in drug resistance in cancer?

UCK2 protein has been associated with drug resistance in cancer cells, particularly in the context of chemotherapy.