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Recombinant Human UBE2I 293 Cell Lysate

Cat.No.: UBE2I-573HCL
Description: Antigen standard for ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: UBE2I ubiquitin-conjugating enzyme E2I [ Homo sapiens ]
Official Symbol: UBE2I
Synonyms: UBE2I; ubiquitin-conjugating enzyme E2I; ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) , ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast); SUMO-conjugating enzyme UBC9; UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); P18; C358B7.1;
Gene ID: 7329
mRNA Refseq: NM_003345
Protein Refseq: NP_003336
MIM: 601661
UniProt ID: P63279
Chromosome Location: 16p13.3
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem;

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