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Recombinant Human TXNIP 293 Cell Lysate

Cat.No.: TXNIP-620HCL
Description: Antigen standard for thioredoxin interacting protein (TXNIP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TXNIP thioredoxin interacting protein [ Homo sapiens ]
Official Symbol: TXNIP
Synonyms: TXNIP; thioredoxin interacting protein; thioredoxin-interacting protein; EST01027; HHCPA78; THIF; thioredoxin binding protein 2; upregulated by 1; 25 dihydroxyvitamin D 3; VDUP1; thioredoxin-binding protein 2; vitamin D3 up-regulated protein 1; upregulated by 1,25-dihydroxyvitamin D-3;
Gene ID: 10628
mRNA Refseq: NM_006472
Protein Refseq: NP_006463
MIM: 606599
UniProt ID: Q9H3M7
Chromosome Location: 1q11
Pathway: Immune System, organism-specific biosystem; Inflammasomes, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways, organism-specific biosystem; The NLRP3 inflammasome, organism-specific biosystem;

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