Recombinant Human TTRAP 293 Cell Lysate TDP2-664HCL

Recombinant Human TTRAP 293 Cell Lysate

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Recombinant Human TTRAP 293 Cell Lysate

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Cat.No. : TDP2-664HCL
Description : Antigen standard for TRAF and TNF receptor associated protein (TTRAP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TDP2 tyrosyl-DNA phosphodiesterase 2 [ Homo sapiens ]
Official Symbol : TDP2
Synonyms : TDP2; tyrosyl-DNA phosphodiesterase 2; TRAF and TNF receptor associated protein , TTRAP; ETS1-associated protein 2; ETS1-associated protein II; tyr-DNA phosphodiesterase 2; 5-Tyr-DNA phosphodiesterase; 5-tyrosyl-DNA phosphodiesterase; TRAF and TNF receptor associated protein; TRAF and TNF receptor-associated protein; EAP2; AD022; EAPII; TTRAP; dJ30M3.3; RP1-30M3.3; MGC9099; MGC111021;
Gene ID : 51567
mRNA Refseq : NM_016614
Protein Refseq : NP_057698
MIM : 605764
UniProt ID : O95551
Chromosome Location : 6p22.3-p22.1
Pathway : CD40/CD40L signaling, organism-specific biosystem;

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