"TIMM50" Related Products

Recombinant Human TIMM50 293 Cell Lysate

Cat.No.: TIMM50-1066HCL
Description: Antigen standard for translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) (TIMM50), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: TIMM50
Synonyms: TIMM50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); translocase of inner mitochondrial membrane 50 homolog (yeast); mitochondrial import inner membrane translocase subunit TIM50; TIM50L; Tim50-like protein; homolog of yeast Tim50; TIM50; MGC102733;
Gene ID: 92609
mRNA Refseq: NM_001001563
Protein Refseq: NP_001001563
MIM: 607381
UniProt ID: Q3ZCQ8
Chromosome Location: 19q13.2
Pathway: Metabolism of proteins, organism-specific biosystem; Mitochondrial Protein Import, organism-specific biosystem;

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