"TACO1" Related Products

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Recombinant Human TACO1 293 Cell Lysate

Cat.No.: TACO1-1284HCL
Description: Antigen standard for translational activator of mitochondrially encoded cytochrome c oxidase I (TACO1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TACO1 translational activator of mitochondrially encoded cytochrome c oxidase I [ Homo sapiens ]
Official Symbol: TACO1
Synonyms: TACO1; translational activator of mitochondrially encoded cytochrome c oxidase I; CCDC44, coiled coil domain containing 44; translational activator of cytochrome c oxidase 1; clone HQ0477 PRO0477p; coiled-coil domain-containing protein 44; CCDC44;
Gene ID: 51204
mRNA Refseq: NM_016360
Protein Refseq: NP_057444
MIM: 612958
UniProt ID: Q9BSH4
Chromosome Location: 17q23.3

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