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Recombinant Human STAT5A 293 Cell Lysate

Cat.No.: STAT5A-1416HCL
Description: Antigen standard for signal transducer and activator of transcription 5A (STAT5A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: STAT5A signal transducer and activator of transcription 5A [ Homo sapiens ]
Official Symbol: STAT5A
Synonyms: STAT5A; signal transducer and activator of transcription 5A; STAT5; MGF;
Gene ID: 6776
mRNA Refseq: NM_003152
Protein Refseq: NP_003143
MIM: 601511
UniProt ID: P42229
Chromosome Location: 17q11.2
Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adipogenesis, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chronic myeloid leukemia, organism-specific biosystem;

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